4-Nitrophenyl α-D-maltopentaoside
4-Nitrophenyl α-D-maltopentaoside (CAS: 66068-38-0) is a synthetic glycoside with the molecular formula C36H55NO28 and a molecular weight of 949.81 g/mol. This compound serves as a chromogenic substrate for the detection and characterisation of amylase activity. It is widely employed in biochemical research and diagnostics, facilitating the quantitative analysis of enzyme kinetics and screening for enzyme inhibitors.
- IUPAC
- 4-Nitrophenyl α-D-penta-(1→4)-glucopyranoside
- Synonyms
- 4-Nitrophenyl α-D-penta-(1→4)-glucopyranoside
Enzyme Substrate
4-Nitrophenyl α-D-maltopentaoside is utilised as a specific chromogenic substrate for alpha-amylase. Upon enzymatic hydrolysis, it releases 4-nitrophenol, which can be quantified spectrophotometrically, allowing for the measurement of enzyme activity.
Biochemical Research
This compound is valuable in glycomics and glycobiology research for studying carbohydrate-protein interactions and enzymatic pathways involving starch degradation. It aids in the characterisation of amylases from various sources.
Diagnostic Assays
It finds application in the development of diagnostic kits for detecting amylase levels, which can be indicative of certain physiological conditions. The reliable release of a coloured product enables straightforward assay design.
Enzyme Inhibition Studies
Researchers use 4-Nitrophenyl α-D-maltopentaoside to screen for and characterise inhibitors of amylase. The reduction in 4-nitrophenol release in the presence of an inhibitor provides a quantitative measure of inhibitory potency.
| Molecular weight | 949.81 |
|---|---|
| Empirical formula | C36H55NO28 |
| Assay | ≥98% |
| Storage temperature | −20°C |
Hazard statements
- H302Harmful if swallowed
- H312Harmful in contact with skin
- H315Causes skin irritation
- H319Causes serious eye irritation
- H332Harmful if inhaled
- H335May cause respiratory irritation
Precautionary statements
- P261Avoid breathing dust, fume, gas or vapours
- P280Wear protective gloves, clothing and eye/face protection
- P305IF IN EYES
| Protective equipment | dust mask type N95 (US), Eyeshields, Gloves |
|---|---|
| Water hazard class (WGK, DE) | 3 |
| Hazard codes (EU) | Xn |
| Risk statements (R) | 20/21/22-36/37/38 |
| Safety statements (S) | 26-36 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is 4-Nitrophenyl α-D-maltopentaoside used for?
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4-Nitrophenyl α-D-maltopentaoside is primarily used as a chromogenic substrate for the assay of amylase activity in biochemical research, diagnostics, and enzyme inhibition studies.
What are the CAS number and formula for 4-Nitrophenyl α-D-maltopentaoside?
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The CAS number for 4-Nitrophenyl α-D-maltopentaoside is 66068-38-0, and its molecular formula is C36H55NO28.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies 4-Nitrophenyl α-D-maltopentaoside with an assay of ≥98%. TSS does not represent this product as USP, BP, EP, or any other pharmacopoeia grade.
How should 4-Nitrophenyl α-D-maltopentaoside be handled safely?
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Handle with appropriate personal protective equipment, including a dust mask (type N95 US recommended), eyeshields, and gloves. Avoid breathing dust. This compound is classified as harmful if swallowed, in contact with skin, or if inhaled, and causes skin and serious eye irritation. Ensure good ventilation. Store at -20°C.
How is 4-Nitrophenyl α-D-maltopentaoside packed and shipped?
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This product is available for global export and is typically supplied in quantities suitable for laboratory research. Specific packaging details are available upon request.
How can I request a quote or sample?
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To request a quote or a sample of 4-Nitrophenyl α-D-maltopentaoside, please contact the Tech Serve Solutions sales team via our website or direct inquiry.
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