4-Nitrophenyl α-D-glucopyranoside
4-Nitrophenyl α-D-glucopyranoside (CAS: 3767-28-0, Formula: C12H15NO8, Molar Mass: 301.25 g/mol) is a PREMIUM quality biochemical reagent. It serves as a chromogenic substrate for various enzymes, particularly alpha-glucosidase. This compound is essential in biochemical research for enzyme activity assays and detection methods, enabling quantitative analysis through the release of a coloured product upon enzymatic hydrolysis. It is widely used in molecular biology and glycobiology studies.
- IUPAC
- p-Nitrophenyl α-D-glucopyranoside

Enzyme Activity Assays
4-Nitrophenyl α-D-glucopyranoside is a key chromogenic substrate used to measure the activity of alpha-glucosidase and related enzymes. Upon enzymatic cleavage, it releases p-nitrophenol, which can be quantified spectrophotometrically.
Biochemical Research
It is employed in various research settings, including glycobiology and molecular biology, to study enzymatic reactions and pathways. Its use facilitates the understanding of post-translational modifications and proteomic analyses involving glycosylation.
Diagnostic Applications
While not a medical diagnostic agent itself, its principle of chromogenic detection can be adapted for diagnostic kits that rely on enzymatic activity measurements.
| Molecular weight | 301.25 |
|---|---|
| Empirical formula | C12H15NO8 |
| Assay | ≥99% |
| Storage temperature | −20°C |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Quality Level | PREMIUM |
|---|
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is 4-Nitrophenyl α-D-glucopyranoside used for?
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4-Nitrophenyl α-D-glucopyranoside is primarily used as a chromogenic substrate for the assay of alpha-glucosidase activity and in biochemical research related to glycobiology and molecular biology.
What is the CAS number and formula for 4-Nitrophenyl α-D-glucopyranoside?
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The CAS number is 3767-28-0, and its molecular formula is C12H15NO8.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies 4-Nitrophenyl α-D-glucopyranoside at PREMIUM quality with an assay of ≥99%. We do not represent this product as USP, BP, or EP grade.
How should 4-Nitrophenyl α-D-glucopyranoside be handled safely?
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Safety precautions include wearing eyeshields and gloves. A type N95 (US) or type P1 (EN143) respirator filter should be used if necessary. The substance has a WGK Germany rating of 3, indicating it is hazardous to water.
How is 4-Nitrophenyl α-D-glucopyranoside packed and shipped?
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This product is typically stored at -20°C. TSS exports fine chemicals globally, ensuring appropriate packaging for international shipment.
How can I request a sample or quote for 4-Nitrophenyl α-D-glucopyranoside?
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Please contact our sales team via the website or your usual representative to request a sample or a formal quotation for 4-Nitrophenyl α-D-glucopyranoside.
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