2′,7′-Dichlorofluorescin diacetate
2′,7′-Dichlorofluorescin diacetate (CAS 4091-99-0) is a cell-permeable, non-fluorescent probe. Upon intracellular de-esterification and subsequent oxidation, it generates highly fluorescent 2′,7′-dichlorofluorescein. This compound is valuable for sensitive and rapid quantitation of reactive oxygen species, playing a crucial role in research related to oxidative metabolism and cell stress.
- IUPAC
- 2′,7′-Dichlorodihydrofluorescein diacetate
- Synonyms
- 2′,7′-Dichlorodihydrofluorescein diacetateDichlorodihydrofluorescein diacetateDCFH-DA
Reactive Oxygen Species Detection
Serves as a sensitive probe for the quantitation of oxygen-reactive species generated during oxidative metabolism. Its fluorescence increases upon oxidation, allowing for real-time monitoring.
Phagocytic Cell Assays
Enables microplate assays for detecting oxidative products within phagocytic cells, aiding in the study of cellular immune responses.
Myeloid Differentiation Studies
Applicable in quantitative multiwell assays for studying myeloid differentiation processes.
Oxidative Stress Research
A key reagent in the investigation of oxidative stress, allowing researchers to measure the generation of reactive oxygen species in various biological systems.
| Molecular weight | 487.29 |
|---|---|
| Empirical formula | C24H16Cl2O7 |
| Assay | ≥97% |
| Storage temperature | −20°C |
| Application | 2′,7′-Dichlorofluorescin diacetate is a cell-permeable non-fluorescent probe. 2′,7′-Dichlorofluorescin diacetate is de-esterified intracellularly and turns to highly fluorescent 2′,7′-dichlorofluorescein upon oxidation.Applications include sensitive and rapid quantitation of oxygen-reactive species in response to oxidative metabolism; microplate assay for detecting oxidative products in phagocytic cells, and quantitative multiwell myeloid differentiation assay. |
Hazard statements
- H315Causes skin irritation
- H319Causes serious eye irritation
- H335May cause respiratory irritation
Precautionary statements
- P261Avoid breathing dust, fume, gas or vapours
- P305IF IN EYES
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
| Hazard codes (EU) | Xi |
| Risk statements (R) | 36/37/38 |
| Safety statements (S) | 26 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is 2′,7′-Dichlorofluorescin diacetate used for?
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2′,7′-Dichlorofluorescin diacetate is used as a cell-permeable probe to detect and quantitate oxygen-reactive species, particularly in the study of oxidative metabolism, phagocytic cell activity, and myeloid differentiation.
What is the CAS number and formula for 2′,7′-Dichlorofluorescin diacetate?
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The CAS number is 4091-99-0 and the empirical formula is C24H16Cl2O7.
What grade and purity does Tech Serve Solutions supply for 2′,7′-Dichlorofluorescin diacetate?
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Tech Serve Solutions supplies 2′,7′-Dichlorofluorescin diacetate with an assay of ≥97%. This product is intended for research use and is not represented as USP, BP, EP, or pharmaceutical grade.
How should 2′,7′-Dichlorofluorescin diacetate be handled safely?
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2′,7′-Dichlorofluorescin diacetate is a Warning substance (GHS07) that causes skin irritation (H315), serious eye irritation (H319), and may cause respiratory irritation (H335). Handle with appropriate personal protective equipment, including eyeshields, gloves, and a type N95 respirator filter. Ensure adequate ventilation and avoid breathing dust.
How is 2′,7′-Dichlorofluorescin diacetate shipped and exported?
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Tech Serve Solutions is a specialist global supplier and exporter of fine chemicals. Products are carefully packed and exported worldwide in compliance with relevant regulations.
How can I request a sample or quote for 2′,7′-Dichlorofluorescin diacetate?
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To request a sample or quote for 2′,7′-Dichlorofluorescin diacetate, please contact our sales team via the website or by telephone, providing your specific requirements.
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