2′,7′-Dichlorofluorescein
2′,7′-Dichlorofluorescein (CAS: 76-54-0) is an organic compound with the molecular formula C20H10Cl2O5. This orange to red-brown powder serves as a pH-sensitive fluorescent indicator, particularly useful as an adsorption indicator in analytical chemistry. Its ability to fluoresce under specific pH conditions makes it valuable for detecting endpoints in titrations and for various biological staining applications.
- Synonyms
- 2′,7′-Dichlorofluorescein2,7-DichlorofluoresceinDichlorofluorescein
Adsorption Indicator
2′,7′-Dichlorofluorescein is employed as an adsorption indicator in argentometric titrations. It demonstrates a distinct color change at the equivalence point, aiding in precise endpoint determination.
Fluorescent Staining
As a fluorescent probe, this compound can be utilised in microscopy and histology for differential staining of cellular structures. Its pH sensitivity allows for visualization of specific cellular environments.
Analytical Reagent
This chemical serves as a versatile reagent in various analytical procedures. Its fluorescent properties are exploited in quantitative analysis and detection methods where pH variations are critical.
| Molecular weight | 401.20 |
|---|---|
| Empirical formula | C20H10Cl2O5 |
| Grade | ACS reagent |
| Colour | orange to red-brown, powder |
| Density | 0.79 g/mL at 20 °C |
| Solubility | alcohol: passes test |
| Suitability | passes test for adsorption indicator |
| Melting point | 280 °C (dec.)(lit.) |
Hazard statements
- H315Causes skin irritation
- H319Causes serious eye irritation
- H335May cause respiratory irritation
Precautionary statements
- P261Avoid breathing dust, fume, gas or vapours
- P305IF IN EYES
| Protective equipment | dust mask type N95 (US), Eyeshields, Gloves |
|---|---|
| Water hazard class (WGK, DE) | 3 |
| Hazard codes (EU) | Xi |
| Risk statements (R) | 36/37/38 |
| Safety statements (S) | 26-36 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Absorption | λmax 509 nm |
|---|
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is 2′,7′-Dichlorofluorescein used for?
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2′,7′-Dichlorofluorescein is primarily used as a pH-sensitive fluorescent indicator, particularly as an adsorption indicator in titrations and for fluorescent staining in biological applications.
What is the CAS number and formula for 2′,7′-Dichlorofluorescein?
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The CAS number for 2′,7′-Dichlorofluorescein is 76-54-0, and its empirical formula is C20H10Cl2O5.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies 2′,7′-Dichlorofluorescein in ACS reagent grade, suitable for analytical purposes. We do not represent this product as USP, BP, or EP grade.
What are the safety and handling precautions for 2′,7′-Dichlorofluorescein?
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2′,7′-Dichlorofluorescein is a warning substance that can cause skin irritation (H315), serious eye irritation (H319), and respiratory irritation (H335). Recommended personal protective equipment includes a dust mask (type N95), eyeshields, and gloves. Handle in a well-ventilated area.
How is 2′,7′-Dichlorofluorescein packed and exported by TSS?
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Tech Serve Solutions professionally packs and exports chemicals globally, adhering to all relevant regulations to ensure safe transit and delivery.
How can I request a sample or quote for 2′,7′-Dichlorofluorescein?
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To request a sample or quote for 2′,7′-Dichlorofluorescein, please contact our sales team through the website or by phone.
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