2′,7′-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein
2′,7′-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein (CAS 85138-49-4), also known as BCECF, is a fluorescent indicator used for intracellular pH measurements. With a molecular weight of 520.44 g/mol, this fluorescein derivative exhibits pH-dependent fluorescence, making it valuable in biological research. Its applications span cell biology and neuroscience, particularly in studying ion channel activity and cell signaling pathways, providing researchers with a reliable tool for monitoring cellular environments.
- IUPAC
- BCECF
- Synonyms
- BCECF2′,7′-Bis(2-carboxyethyl)-2,7-difluoroscein-5(6)-carboxylic acid
Intracellular pH Monitoring
BCECF is widely employed as a fluorescent indicator for measuring intracellular pH (pHi) in living cells. Its fluorescence properties change significantly with pH, allowing for real-time quantitative analysis of cellular acidity and alkalinity.
Ion Channel Research
This compound is instrumental in studying the activity of various ion channels, including voltage-gated and monovalent channels. By monitoring pH changes, researchers can infer the function and regulation of these critical membrane proteins.
Cell Signaling Studies
BCECF aids in understanding complex cell signaling cascades where pH fluctuations play a crucial role. It is particularly useful in neuroscience research to investigate neuronal activity and excitability.
Biochemical Assays
As a fluorescent probe, BCECF can be utilized in various biochemical assays to detect and quantify cellular processes that involve changes in proton concentration, contributing to a broader understanding of cellular metabolism.
| Molecular weight | 520.44 |
|---|---|
| Empirical formula | C27H20O11 |
| Assay | ≥90% (HPLC) |
| Storage temperature | −20°C |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is 2′,7′-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein used for?
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2′,7′-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein (CAS 85138-49-4), also known as BCECF, is primarily used as a fluorescent indicator for measuring intracellular pH (pHi) in living cells, and in research related to ion channels and cell signaling.
What are the CAS number and formula for 2′,7′-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein?
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The CAS number for 2′,7′-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein is 85138-49-4. Its empirical formula is C27H20O11, and its molecular weight is 520.44 g/mol.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies 2′,7′-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein with an assay of ≥90% (HPLC). This product is intended for research and laboratory use. Tech Serve Solutions does not represent this product as USP, BP, EP, or any pharmacopoeia grade.
How should 2′,7′-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein be handled safely?
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Handling requires appropriate personal protective equipment, including eyeshields and gloves. A type N95 (US) or type P1 (EN143) respirator filter is recommended. The product has a WGK Germany classification of 3, indicating it is hazardous to water. Avoid release into the environment.
How is 2′,7′-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein packed and shipped?
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This chemical is typically stored at −20°C. For shipping and export, it is carefully packaged to maintain its integrity and comply with all relevant regulations for chemical transport. Please contact us for specific shipping details.
How can I request a sample or quote for 2′,7′-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein?
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To request a sample or a quote for 2′,7′-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein, please use the 'Request a Quote' or 'Sample Request' form on our website, or contact our sales team directly via email or phone.
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