2′,7′-Dichlorofluorescein diacetate
2′,7′-Dichlorofluorescein diacetate (CAS: 2044-85-1), with a molecular formula of C24H14Cl2O7 and a molecular weight of 485.27, is a vital biochemical reagent and fluorescent probe. This derivative of fluorescein is specifically designed for fluorescence applications, offering distinct excitation and emission wavelengths. Its suitability for fluorescence makes it a valuable tool in various analytical and research settings requiring sensitive detection methods.
- Synonyms
- 2′,7′-Dichlorofluorescein diacetate
Fluorescent Labelling
Utilised as a fluorescent label in biological and chemical assays due to its distinct spectral properties. The compound's fluorescence characteristics allow for sensitive detection and quantification in various experimental setups.
Esterase Activity Assays
Serves as a substrate for detecting esterase activity, where enzymatic hydrolysis of the acetate groups releases the fluorescent dichlorofluorescein. The resulting fluorescence intensity is proportional to the esterase activity present.
Cellular Imaging and Staining
Employed in cellular studies for imaging and staining purposes, where its fluorescence can be used to visualise specific cellular components or processes after suitable activation or localisation.
| Molecular weight | 485.27 |
|---|---|
| Empirical formula | C24H14Cl2O7 |
| Assay | ≥95% (HPLC) |
| Solubility | DMSO: soluble |
| Storage temperature | 2-8°C |
| Application | Do you have application information on this product that you would like to share? |
| Suitability | suitable for fluorescence |
| Melting point | 232-234 °C(lit.) |
| Protective equipment | dust mask type N95 (US), Eyeshields, Gloves |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Product Line | BioReagent |
|---|---|
| Fluorescence | λex 504 nm; λem 524 nm in 0.1 M Tris pH 8.0 (esterase) |
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is 2′,7′-Dichlorofluorescein diacetate used for?
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2′,7′-Dichlorofluorescein diacetate is primarily used as a fluorescent probe and label in biochemical research, particularly for detecting esterase activity and in cellular imaging applications.
What is the CAS number and molecular formula for 2′,7′-Dichlorofluorescein diacetate?
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The CAS number is 2044-85-1 and the molecular formula is C24H14Cl2O7.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies 2′,7′-Dichlorofluorescein diacetate with an assay of ≥95% (HPLC). We do not represent this product as USP, BP, or EP grade.
How should 2′,7′-Dichlorofluorescein diacetate be handled safely?
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When handling 2′,7′-Dichlorofluorescein diacetate, it is essential to use personal protective equipment including a dust mask (type N95), eyeshields, and gloves. The compound is classified under WGK 2 in Germany, indicating it is hazardous to water.
How is 2′,7′-Dichlorofluorescein diacetate shipped and exported?
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Tech Serve Solutions, established in 1998, is a specialist global supplier and exporter of fine chemicals, including 2′,7′-Dichlorofluorescein diacetate, adhering to all relevant shipping and export regulations.
How can I request a quote or sample for this product?
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To request a quote or sample of 2′,7′-Dichlorofluorescein diacetate, please contact our sales team through the provided channels on the Tech Serve Solutions website.
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