ZnAF-2F
ZnAF-2F (CAS: 443302-09-8) is a difluorofluorescein derivative with the molecular formula C34H26F2N4O5 and a molecular weight of 608.59. This fluorescent indicator is specifically designed for the fluorimetric detection of Zn2+ ions. Its utility lies in its ability to exhibit fluorescence changes upon binding with zinc, making it a valuable tool in biochemical research and analytical chemistry for quantifying zinc levels.
- IUPAC
- 6-{2-[Bis(2-pyridylmethyl)amino]ethylamino}-2′,7′-difluorofluorescein
- Synonyms
- 6-{2-[Bis(2-pyridylmethyl)amino]ethylamino}-2′,7′-difluorofluorescein

Fluorimetric Detection of Zinc Ions
ZnAF-2F is employed as a fluorescent probe for the sensitive and selective detection of Zn2+ in various biological and chemical samples. It allows for real-time monitoring of zinc ion concentrations.
Biochemical Research
This compound serves as a reagent in biochemical studies to investigate the role of zinc in cellular processes. Its fluorescence properties aid in understanding zinc homeostasis and signalling pathways.
Analytical Chemistry
Used as an indicator in analytical methods requiring the quantification of zinc ions. The excitation and emission wavelengths (λex 492 nm; λem 517 nm) are key parameters for its application in fluorescence spectroscopy.
| Molecular weight | 608.59 |
|---|---|
| Empirical formula | C34H26F2N4O5 |
| Assay | ≥95.0% (HPCE) |
| Storage temperature | −20°C |
| Application | suitable for the fluorimetric detection of Zn2+,1 |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Fluorescence | λex 492 nm; λem 517 nm in PBS, Zn2+ |
|---|
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is ZnAF-2F used for?
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ZnAF-2F is used for the fluorimetric detection of Zn2+ ions, serving as a valuable tool in biochemical research and analytical chemistry for quantifying zinc levels.
What are the CAS number and molecular formula for ZnAF-2F?
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The CAS number for ZnAF-2F is 443302-09-8, and its molecular formula is C34H26F2N4O5.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies ZnAF-2F with a purity of ≥95.0% (HPCE). This product is intended for research and laboratory use and is not represented as USP, BP, EP, or pharmacopoeia grade.
How should ZnAF-2F be handled and stored?
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Handle ZnAF-2F with appropriate personal protective equipment, including eyeshields and gloves. A type N95 (US) or type P1 (EN143) respirator filter is recommended. Store at -20°C.
Is ZnAF-2F a hazardous substance?
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ZnAF-2F is classified with a WGK Germany rating of 3, indicating it is a hazardous substance. Appropriate safety precautions must be taken during handling and use.
How can I order ZnAF-2F or request a quote?
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You can request a quote or inquire about ordering ZnAF-2F through the Tech Serve Solutions website or by contacting our sales team. Samples may also be requested via our online portal.
Can I get a sample of ZnAF-2F?
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Yes, samples of ZnAF-2F can be requested through the Tech Serve Solutions website. Please visit our sample request section for details.
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