ZnAF-2 DA
ZnAF-2 DA (CAS 357339-96-9), with the empirical formula C38H32N4O7 and a molecular weight of 656.68, is a specialised biochemical reagent. It functions as a fluorescent probe designed for the fluorimetric detection of Zn<sup>2+</sup> ions. Its utility is realised after hydrolysis by esterase enzymes, which cleaves the acetate groups, enabling fluorescence emission. This compound is primarily employed in biological research and assay development.
- IUPAC
- 6-{2-[Bis(2-pyridylmethyl)amino]ethylamino}fluorescein diacetate
- Synonyms
- 6-{2-[Bis(2-pyridylmethyl)amino]ethylamino}fluorescein diacetate
Fluorimetric Detection of Zinc Ions
ZnAF-2 DA is specifically designed for the sensitive fluorimetric detection of Zn<sup>2+</sup>. The probe's fluorescence is activated upon esterase-mediated hydrolysis, making it suitable for biological samples where intracellular esterase activity is present.
Zinc Ion Research
This compound serves as a valuable tool in studies investigating the role of zinc ions in biological processes. Its fluorescent properties allow for real-time monitoring of zinc fluctuations in cellular environments.
Assay Development
Utilised in the development of biochemical assays requiring the quantification of zinc ions. The probe's sensitivity and fluorescence characteristics aid in establishing robust detection methods.
| Molecular weight | 656.68 |
|---|---|
| Empirical formula | C38H32N4O7 |
| Assay | ~90% (HPCE) |
| Storage temperature | 2-8°C |
| Application | Suitable for the fluorimetric detection of Zn2+ (after esterase hydrolysis). |
Hazard statements
- H413May cause long-lasting harmful effects to aquatic life
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Fluorescence | λex 492 nm; λem 515 nm in PBS, Zn2+, esterase |
|---|
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is ZnAF-2 DA used for?
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ZnAF-2 DA is used for the fluorimetric detection of Zn<sup>2+</sup> ions after esterase hydrolysis, serving as a fluorescent probe in biological research and assay development.
What is the CAS number and formula for ZnAF-2 DA?
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The CAS number for ZnAF-2 DA is 357339-96-9, and its empirical formula is C38H32N4O7.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies ZnAF-2 DA with an assay of approximately 90% (HPCE). We do not represent this product as USP, BP, EP, or other pharmacopoeia grades.
How should ZnAF-2 DA be handled and stored?
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When handling ZnAF-2 DA, appropriate personal protective equipment, including eyeshields, gloves, and a type N95 (US) or type P1 (EN143) respirator filter, should be used. The compound is classified under WGK 2 and has hazard statement H413. Store at 2-8°C.
How is ZnAF-2 DA packed, shipped, and exported?
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ZnAF-2 DA is carefully packed and prepared for global export by Tech Serve Solutions, adhering to all necessary shipping regulations for chemical compounds.
How can I request a sample or quote for ZnAF-2 DA?
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To request a sample or a quote for ZnAF-2 DA, please contact our sales team through the website or by phone.
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