Pyridoxal hydrochloride
Pyridoxal hydrochloride (CAS 65-22-5), with the formula C8H9NO3 · HCl and a molecular weight of 203.62 g/mol, is a vital form of Vitamin B6. This aldehyde compound serves as a crucial coenzyme in numerous metabolic reactions, particularly in amino acid metabolism. Its primary application lies in the precise labelling and detection of amino acids in biochemical research, enabling quantification at picomolar levels. It is a key reagent in cell biology and chemical synthesis.
- IUPAC
- 3-Hydroxy-5-(hydroxymethyl)-2-methyl-4-pyridinecarboxaldehyde hydrochloride,PL HCl
- Synonyms
- 3-Hydroxy-5-(hydroxymethyl)-2-methyl-4-pyridinecarboxaldehyde hydrochloride,PL HCl

Amino Acid Labelling and Detection
Pyridoxal hydrochloride is employed for the specific labelling of amino acids. This enables their sensitive detection and quantification, even at very low concentrations such as picomolar amounts in biochemical assays.
Coenzyme and Cofactor Research
As a form of Vitamin B6, pyridoxal hydrochloride functions as a crucial coenzyme in various enzymatic reactions. It is instrumental in studies related to nutrition research and understanding metabolic pathways.
Biochemical and Cell Biology Applications
This compound is widely used as a biochemical reagent in cell biology and cell culture research. It plays a role in understanding cellular processes and as a building block in chemical synthesis.
| Molecular weight | 203.62 |
|---|---|
| Empirical formula | C8H9NO3 · HCl |
| Assay | ≥99.5% (T) |
| Loss on drying | ≤0.6% loss on drying |
| Storage temperature | −20°C |
| Application | For the labeling of amino acids and their detection in picomolar amounts1; Coenzymes and Cofactors, vol. 1: Vitamin B6, Pyridoxal Phosphate2 |
| Melting point | 173 °C (dec.)(lit.) |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 2 |
| Cation traces | Ca: ≤250 mg/kgCd: ≤5 mg/kgCo: ≤5 mg/kgCr: ≤15 mg/kgCu: ≤5 mg/kgFe: ≤15 mg/LK: ≤50 mg/kgMg: ≤10 mg/kgMn: ≤100 mg/kgNa: ≤50 mg/kgNi: ≤5 mg/kgPb: ≤5 mg/kgZn: ≤5 mg/kg |
| Anion traces | sulfate (SO42-): ≤1000 mg/kg |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Product Line | BioReagent |
|---|
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is Pyridoxal hydrochloride used for?
+
Pyridoxal hydrochloride is used for the labelling and detection of amino acids in picomolar amounts, and as a coenzyme and cofactor in biochemical studies, particularly related to Vitamin B6 metabolism.
What is the CAS number and formula for Pyridoxal hydrochloride?
+
The CAS number is 65-22-5 and the empirical formula (Hill notation) is C8H9NO3 · HCl.
What grade and purity does Tech Serve Solutions supply?
+
Tech Serve Solutions supplies Pyridoxal hydrochloride with an assay of ≥99.5% (T). TSS does not represent this product as USP, BP, EP, or pharmacopoeia grade unless explicitly stated.
What are the safety considerations for handling Pyridoxal hydrochloride?
+
Pyridoxal hydrochloride is classified as WGK 2. It requires the use of safety precautions such as eyeshields and gloves. A respirator filter of type N95 (US) or P1 (EN143) is recommended.
How is Pyridoxal hydrochloride shipped and exported?
+
Pyridoxal hydrochloride is typically stored at -20°C. Tech Serve Solutions is a specialist global supplier and exporter of fine chemicals, capable of handling international shipments according to regulatory requirements.
How can I request a quote or sample of Pyridoxal hydrochloride?
+
To request a quote or sample of Pyridoxal hydrochloride, please contact our sales team through the website or by phone.
▶ Related products

1-(2-Fluorobenzyl)piperazine
C11H15FN2
Chemical Synthesis
1-(2-Fluorophenyl)biguanide hydrochloride
Chemical Synthesis

1-(2-Fluorophenyl)cyclopropanecarboxylic acid
C10H9FO2
Chemical Synthesis
1-(2-Fluorophenyl)piperazine
C10H13FN2
Chemical Synthesis
1-(2-Fluorophenyl)piperazine monohydrochloride
C10H13FN2 · HCl
Chemical Synthesis
1-(2-Furoyl)-1H-benzotriazole
C11H7N3O2
Chemical Synthesis
1-(2-Furoyl)piperazine
C9H12N2O2
Chemical Synthesis
1-(2-Hydroxy-5-methylphenyl)-3-phenyl-1,3-propanedione
Chemical Synthesis
▶ Explore more

3-Methyl-1,5-di-p-tolyl-1,4-pentazadiene
Chemical Synthesis

3-Methyl-1,5-pentanediol
Chemical Synthesis

3-Methyl-1-(2-methylphenyl)-1H-pyrazol-5-amine
C11H13N3
Chemical Synthesis
3-Methyl-1-(3′-sulfoamidophenyl)-5-pyrazolone
C10H11N3O3S
Chemical Synthesis