o-Tolidine dihydrochloride
O-Tolidine dihydrochloride (CAS: 612-82-8), with the linear formula [-C6H3(CH3)-4-NH2]2 · 2HCl and a molecular weight of 285.21 g/mol, is a vital chemical compound. It functions as a sensitive colorimetric reagent. Its primary applications include the detection of free chlorine in water and as a reagent for gold analysis, serving the needs of analytical chemistry and various industrial testing protocols.
- IUPAC
- 3,3′-Dimethylbenzidine dihydrochloride,4,4′-Bianisidine dihydrochloride,4,4′-Diamino-3,3′-dimethylbiphenyl dihydrochloride
![o-Tolidine dihydrochloride ([-C6H3(CH3)-4-NH2]2 · 2HCl) — chemical structure, CAS 612-82-8; Chemistry, fine chemical supplied by Tech Serve Solutions](/_next/image?url=https%3A%2F%2Fpub-f4d920c906024441a773a96f7098e551.r2.dev%2Fimages%2Fproducts%2F612-82-8.png&w=3840&q=75)
Water Quality Testing
O-Tolidine dihydrochloride serves as a sensitive colorimetric reagent for the determination of free chlorine in water samples. This application is crucial for monitoring water purity and treatment efficacy in municipal and industrial settings.
Gold Analysis
This compound is employed as a reagent in the colorimetric analysis of gold. It enables the quantitative detection of gold ions, supporting applications in metallurgy and precious metal assaying.
Chemical Synthesis
As an organic building block and nitrogen compound, o-Tolidine dihydrochloride can be utilised in various chemical synthesis pathways. Its structure lends itself to the creation of more complex organic molecules.
| Molecular weight | 285.21 |
|---|---|
| Linear formula | [-C6H3(CH3)-4-NH2]2 · 2HCl |
| Assay | ≥99.0% (HPLC) |
| Application | Sensitive colorimetric reagent for gold and for free chlorine in water. |



Hazard statements
- H302Harmful if swallowed
- H350May cause cancer
- H411Toxic to aquatic life with long-lasting effects
Precautionary statements
- P201Obtain special instructions before use
- P273Avoid release to the environment
- P308IF exposed or concerned
| Protective equipment | Eyeshields, Faceshields, full-face particle respirator type N100 (US), Gloves, respirator cartridge type N100 (US), type P1 (EN143) respirator filter, type P3 (EN 143) respirator cartridges |
|---|---|
| Transport (UN / ADR) | UN 3077 9 / PGIII |
| Water hazard class (WGK, DE) | 3 |
| Hazard codes (EU) | T,N |
| Risk statements (R) | 45-22-51/53 |
| Safety statements (S) | 53-45-61 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is O-Tolidine dihydrochloride primarily used for?
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O-Tolidine dihydrochloride is primarily used as a sensitive colorimetric reagent for the detection of free chlorine in water and for the analysis of gold.
What is the CAS number and chemical formula for O-Tolidine dihydrochloride?
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The CAS number is 612-82-8 and the linear chemical formula is [-C6H3(CH3)-4-NH2]2 · 2HCl.
What grade and purity does Tech Serve Solutions supply for O-Tolidine dihydrochloride?
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Tech Serve Solutions supplies O-Tolidine dihydrochloride with an assay of ≥99.0% (HPLC). TSS does not represent this product as USP, BP, EP, or any other pharmacopoeia grade.
What are the key safety considerations when handling O-Tolidine dihydrochloride?
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O-Tolidine dihydrochloride is classified under GHS07, GHS08, and GHS09, with the signal word 'Danger'. It carries hazard statements H302 (Harmful if swallowed), H350 (May cause cancer), and H411 (Toxic to aquatic life with long lasting effects). Appropriate personal protective equipment, including eyeshields, gloves, and respiratory protection (type N100 or P3), is essential. Handle in well-ventilated areas and avoid release to the environment. Always consult the Safety Data Sheet (SDS) prior to use.
How is O-Tolidine dihydrochloride typically packed and shipped?
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O-Tolidine dihydrochloride is shipped as UN 3077, Hazard Class 9, Packing Group III, signifying it as an Environmentally Hazardous Substance, solid, n.o.s. Packaging adheres to international regulations for safe transport.
How can I request a quote or sample of O-Tolidine dihydrochloride?
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To request a quote or a sample, please contact the Tech Serve Solutions sales team directly through our website or by phone. Our representatives will be pleased to assist you with your specific requirements.
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