o-Phenylenediamine dihydrochloride
o-Phenylenediamine dihydrochloride (CAS: 615-28-1), with the linear formula C6H4(NH2)2·2HCl and a molecular weight of 181.06, is a vital organic building block. This compound serves as a sensitive peroxidase substrate, particularly valuable in Enzyme-Linked Immunosorbent Assay (ELISA) procedures. It facilitates the detection of enzymatic activity through a colorimetric reaction, yielding a soluble orange-brown product.
ELISA Peroxidase Substrate
o-Phenylenediamine dihydrochloride is employed as a substrate for peroxidases in ELISA. It produces a soluble, orange-brown end product that can be quantified spectrophotometrically at 450 nm, enabling sensitive detection in biochemical assays.
Chemical Synthesis Intermediate
As a diamine derivative, this compound acts as a versatile building block in organic synthesis. It is used in the preparation of various heterocyclic compounds and other complex organic molecules.
Chromogenic Reagent
The compound is utilised as a chromogenic reagent due to its ability to form a coloured product upon enzymatic oxidation. This property is exploited in various analytical and diagnostic applications.
| Molecular weight | 181.06 |
|---|---|
| Linear formula | C6H4(NH2)2·2HCl |
| Assay | ≥99.0% (AT) |
| Storage temperature | 2-8°C |
| Application | o-Phenylenediamine is a peroxidase substrate suitable for use in ELISA procedures. The substrate produces a soluble end product that is orange-brown in color and can be read spectrophotometrically at 450 nm. The OPD reaction may be stopped with 3 N HCl or 3 M H2SO4 and read at 492 nm. |
| Melting point | 258 °C (dec.)(lit.) |
Hazard statements
- H301Toxic if swallowed
- H312Harmful in contact with skin
- H317May cause an allergic skin reaction
- H319Causes serious eye irritation
- H332Harmful if inhaled
- H341Suspected of causing genetic defects
- H351Suspected of causing cancer
- H410Very toxic to aquatic life with long-lasting effects
Precautionary statements
- P273Avoid release to the environment
- P280Wear protective gloves, clothing and eye/face protection
- P301IF SWALLOWED
- P305IF IN EYES
- P501Dispose of contents and container per local regulations
| Protective equipment | dust mask type N95 (US), Eyeshields, Faceshields, Gloves, type P2 (EN 143) respirator cartridges |
|---|---|
| Transport (UN / ADR) | UN 1673 6.1 / PGIII |
| Water hazard class (WGK, DE) | 3 |
| Hazard codes (EU) | T,N |
| Risk statements (R) | 20/21-25-36-40-43-50/53-68 |
| Safety statements (S) | 28-36/37-45-60-61 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is o-Phenylenediamine dihydrochloride used for?
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o-Phenylenediamine dihydrochloride is primarily used as a peroxidase substrate in ELISA procedures and as a building block in chemical synthesis for creating heterocyclic compounds and other organic molecules.
What is the CAS number and formula for o-Phenylenediamine dihydrochloride?
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The CAS number is 615-28-1, and the linear formula is C6H4(NH2)2·2HCl.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies o-Phenylenediamine dihydrochloride with an assay of ≥99.0% (AT). We do not represent this product as USP, BP, or EP grade.
How should o-Phenylenediamine dihydrochloride be handled safely?
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Handle with appropriate personal protective equipment, including a dust mask type N95, eyeshields, faceshields, gloves, and P2 respirator cartridges. It is toxic if swallowed or inhaled, harmful in contact with skin, causes skin irritation, serious eye irritation, and may cause an allergic skin reaction. It is also suspected of causing genetic defects, cancer, and is very toxic to aquatic life with long-lasting effects.
How is o-Phenylenediamine dihydrochloride packed, shipped, and exported?
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This chemical is packed according to industry standards for safe transport and exported globally by Tech Serve Solutions. Specific packaging details are available upon request, adhering to UN 1673 regulations for hazardous goods.
How can I request a sample or quote?
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To request a sample or a quote for o-Phenylenediamine dihydrochloride, please contact our sales team through the website or by phone, providing your specific requirements.
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