Nile Blue A

CAS 3625-57-82C20H20N3O · SO4Cell Biology

Nile Blue A (CAS 3625-57-8), a certified stain with the formula 2C20H20N3O · SO4, is a vital laboratory reagent. This oxazine dye is known for its differential staining capabilities, making it invaluable in biological and histological applications. Its primary use involves visualising cellular structures, particularly mitochondria and nuclei, aiding in research and diagnostics. Supplied by TSS, Nile Blue A meets stringent quality standards for reliable experimental outcomes.

IUPAC: Basic Blue 12,Nile blue sulfate
Synonyms: Nile blue sulfateBasic Blue 12

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Nile Blue A (2C20H20N3O · SO4) — chemical structure, CAS 3625-57-8; Life Science, fine chemical supplied by Tech Serve Solutions

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01 /Applications

Biological Staining

Nile Blue A is widely employed as a vital stain for visualising cellular components, especially nuclei and mitochondria. Its ability to differentiate between cell viability and non-viability is crucial in many biological assays.

Histology and Cytology

In histological preparations, this certified stain is used for differentiating cellular structures. It aids in the detailed examination of tissue morphology and cellular integrity in cytological studies.

Fluorescence Microscopy

Exhibiting fluorescence at specific wavelengths, Nile Blue A is suitable for applications in fluorescence microscopy, enabling advanced imaging of biological samples.

02 /Properties

Molecular weight	732.85
Linear formula	2C20H20N3O · SO4
Grade	certified by the Biological Stain Commission
Composition	Dye content, ≥75%
Melting point	>300 °C (dec.)(lit.)

03 /Safety & handling

Protective equipment	Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
Water hazard class (WGK, DE)	3

Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.

04 /Identifiers & registry

CAS number

3625-57-8

MDL number

MFCD00064529

PubChem substance

24897446

05 /Additional specifications

Colour Index Number	51180
Absorption	λmax 633 nm

06 /Quality & supply

Documentation

Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.

Supply & logistics

Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.

07 /Frequently asked questions

What is Nile Blue A used for?

Nile Blue A is primarily used as a vital stain in cell biology, histology, and cytology for visualising cellular structures like nuclei and mitochondria, and for assessing cell viability. It is also used in fluorescence microscopy.

What is the CAS number and chemical formula for Nile Blue A?

The CAS number for Nile Blue A is 3625-57-8, and its chemical formula is 2C20H20N3O · SO4.

What grade and purity does Tech Serve Solutions supply?

Tech Serve Solutions supplies Nile Blue A as a 'certified by the Biological Stain Commission' grade with a dye content of ≥75%. TSS does not represent this product as USP, BP, EP, or other pharmacopoeia grades unless explicitly stated.

What are the safety and handling recommendations for Nile Blue A?

Nile Blue A is classified with WGK Germany 3. Recommended personal protective equipment includes eyeshields, gloves, and a type N95 (US) or type P1 (EN143) respirator filter. Handle with appropriate caution to avoid exposure.

How is Nile Blue A supplied and exported?

Nile Blue A is available from Tech Serve Solutions, a global supplier and exporter of fine chemicals. Specific packaging and shipping details are provided upon request.