Naphthol AS-MX phosphate disodium salt
Naphthol AS-MX phosphate disodium salt (CAS 96189-12-7, C19H16NO5PNa2) is a chromogenic substrate essential for enzyme detection in histochemistry. With a molecular weight of 415.29, it is primarily utilised in the azo-dye method for visualising phosphatase activity. This compound is also employed in immunohistochemical staining techniques, allowing for the simultaneous detection of different enzymatic activities within biological samples, making it a valuable reagent for cell biology and diagnostic applications.

Phosphatase Detection
Used as a substrate in the histochemical azo-dye method to detect and localise phosphatase enzyme activity in tissue samples. This allows for the visual identification of enzyme-rich areas.
Immunohistochemical Staining
Applied in conjunction with other enzyme systems, such as horseradish peroxidase (POD), for chromogenic double staining techniques. This enables the simultaneous visualisation of multiple cellular markers or enzyme activities.
Cell Biology Research
A key reagent for studying cellular processes involving phosphatase enzymes. Its use in staining methods aids in understanding cell structure and function at a microscopic level.
Histology and Hematology
Valuable in routine histological and hematological staining procedures for identifying specific enzymatic markers in blood cells and tissue sections.
| Molecular weight | 415.29 |
|---|---|
| Linear formula | C19H16NO5PNa2 |
| Assay | ≥98.0% (HPLC) |
| Storage temperature | −20°C |
| Application | Naphthol AS-MX phosphate is a phosphatase substrate used in the histochemical azo-dye method of phosphatase detection.1 Naphthol AS-MX and Fast Blue BB were used for alkaline phosphatase (AP) activity in conjunction with horseradish peroxidase (POD) and 3-amino-9-ethylcarbazole in chromogenic immunohistochemical double staining techniques.2 |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is Naphthol AS-MX phosphate disodium salt used for?
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Naphthol AS-MX phosphate disodium salt is used as a substrate in histochemical azo-dye methods for detecting phosphatase activity and in immunohistochemical double staining techniques.
What are the CAS number and chemical formula?
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The CAS number is 96189-12-7 and the chemical formula is C19H16NO5PNa2.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies Naphthol AS-MX phosphate disodium salt with an assay of ≥98.0% (HPLC). This is a technical/research grade product and is not represented as USP, BP, or EP.
How should Naphthol AS-MX phosphate disodium salt be handled?
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Handle with appropriate personal protective equipment, including eyeshields and gloves. A type N95 (US) or type P1 (EN143) respirator filter is recommended. Store at -20°C.
Is this product a controlled substance?
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Based on the provided data, Naphthol AS-MX phosphate disodium salt is not indicated as a controlled substance.
How is this chemical packed and shipped?
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Tech Serve Solutions is a specialist global supplier and exporter of fine chemicals, ensuring appropriate packaging and compliance with international shipping regulations for this product.
How can I request a sample or quote?
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Please contact Tech Serve Solutions directly via our website or sales department to request a sample or obtain a quotation for Naphthol AS-MX phosphate disodium salt.
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