N-Acetyl-Asp-Glu-Val-Asp-7-amido-4-methylcoumarin

CAS 169332-61-0C30H37N5O13Biochemicals & Reagents

N-Acetyl-Asp-Glu-Val-Asp-7-amido-4-methylcoumarin (CAS: 169332-61-0) is a synthetic fluorogenic substrate for caspases. With the empirical formula C30H37N5O13 and a molecular weight of 675.64, this white powder is primarily utilised in biochemical research for studying enzyme activity, particularly in the context of apoptosis. Its ability to release a fluorescent signal upon cleavage makes it invaluable for quantifying caspase activity in vitro and in cell-based assays.

IUPAC: Ac-DEVD-AMC
Synonyms: Ac-DEVD-AMC

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N-Acetyl-Asp-Glu-Val-Asp-7-amido-4-methylcoumarin (C30H37N5O13) — chemical structure, CAS 169332-61-0; Life Science, fine chemical supplied by Tech Serve Solutions

A-AMApoptosis EnzymesApoptosis ReagentsApoptosis and Cell CycleApplication IndexBioactive Small MoleculesBiochemicals and ReagentsCaspase 3Caspase 7Caspase Substrates

01 /Applications

Caspase Activity Assays

This compound serves as a sensitive fluorogenic substrate for detecting and quantifying the activity of various caspases, crucial enzymes involved in programmed cell death (apoptosis). Its cleavage by specific caspases releases a fluorescent AMC moiety, allowing for real-time monitoring of enzyme kinetics.

Apoptosis Research

As a tool for studying apoptosis, N-Acetyl-Asp-Glu-Val-Asp-7-amido-4-methylcoumarin aids researchers in understanding the intricate signalling pathways that regulate cell death. It can be used to screen for caspase inhibitors or activators and to assess the impact of cellular events on caspase activation.

Cellular Imaging and Screening

The fluorescent properties of the liberated AMC group enable its use in cellular imaging studies and high-throughput screening (HTS) applications. Researchers can visualise and quantify caspase activity within cells or in complex biological samples.

02 /Properties

Molecular weight	675.64
Empirical formula	C30H37N5O13
Assay	≥97% (HPLC)
Form	powder
Colour	white
Solubility	DMSO: soluble10 mM
Storage temperature	−20°C
Composition	Peptide content, ~65%

03 /Safety & handling

Protective equipment	Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
Water hazard class (WGK, DE)	3

Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.

04 /Identifiers & registry

CAS number

169332-61-0

MDL number

MFCD00671411

PubChem substance

24890505

05 /Quality & supply

Documentation

Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.

Supply & logistics

Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.

06 /Frequently asked questions

What is N-Acetyl-Asp-Glu-Val-Asp-7-amido-4-methylcoumarin used for?

N-Acetyl-Asp-Glu-Val-Asp-7-amido-4-methylcoumarin is used as a fluorogenic substrate for the detection and quantification of caspase enzyme activity, particularly in research related to apoptosis and cell signalling.

What is the CAS number and formula for N-Acetyl-Asp-Glu-Val-Asp-7-amido-4-methylcoumarin?

The CAS number is 169332-61-0, and the empirical formula is C30H37N5O13.

What grade and purity does Tech Serve Solutions supply?

Tech Serve Solutions supplies N-Acetyl-Asp-Glu-Val-Asp-7-amido-4-methylcoumarin with an assay of ≥97% (HPLC). This product is intended for research purposes and is not represented as USP, BP, EP, or any other pharmacopoeia grade.

What are the safety and handling recommendations?

Appropriate personal protective equipment, including eyeshields, gloves, and a type N95 (US) or type P1 (EN143) respirator filter, should be used when handling this compound. It is classified with WGK Germany 3, indicating a high hazard level.

How is N-Acetyl-Asp-Glu-Val-Asp-7-amido-4-methylcoumarin shipped and exported?

This chemical is typically stored at -20°C. Tech Serve Solutions is a global supplier and exporter, adhering to all relevant shipping and export regulations for chemical compounds.

How can I request a quote or sample?

To request a quote or a sample of N-Acetyl-Asp-Glu-Val-Asp-7-amido-4-methylcoumarin, please contact our sales department through the website or via direct inquiry.