N-Acetyl-Asp-Glu-Val-Asp-7-amido-4-trifluoromethylcoumarin
N-Acetyl-Asp-Glu-Val-Asp-7-amido-4-trifluoromethylcoumarin (CAS: 201608-14-2) is a fluorogenic substrate with the empirical formula C30H34F3N5O13 and a molecular weight of 729.61 g/mol. Supplied as a white powder, it is soluble in water. This compound is primarily utilised as a research tool in biochemical and cell biology studies, particularly for the detection and quantification of enzyme activity within apoptotic pathways.
- IUPAC
- Ac-DEVD-AFC
- Synonyms
- Ac-DEVD-AFC
Caspase Activity Assays
This substrate is employed for the fluorometric detection of caspases, a family of cysteine proteases crucial for programmed cell death. Its cleavage by specific caspases releases a fluorescent AMC moiety, enabling quantitative analysis of enzyme activity.
Apoptosis Research
As a substrate for caspases involved in apoptosis, N-Acetyl-Asp-Glu-Val-Asp-7-amido-4-trifluoromethylcoumarin aids researchers in investigating cellular mechanisms of programmed cell death and related signalling pathways.
Enzyme Characterisation
The compound serves as a valuable tool for characterising the kinetic properties and substrate specificities of various caspases in vitro, contributing to a deeper understanding of protease function.
| Molecular weight | 729.61 |
|---|---|
| Empirical formula | C30H34F3N5O13 |
| Assay | ≥90% (HPLC) |
| Form | powder |
| Colour | white |
| Solubility | H2O: soluble1 mg/mL |
| Storage temperature | −20°C |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Gene Information | human ... CASP3(836) |
|---|
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is N-Acetyl-Asp-Glu-Val-Asp-7-amido-4-trifluoromethylcoumarin used for?
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N-Acetyl-Asp-Glu-Val-Asp-7-amido-4-trifluoromethylcoumarin is used as a fluorogenic substrate for the detection and quantification of caspase activity, particularly in research concerning apoptosis and cell signalling pathways.
What is the CAS number and formula for this product?
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The CAS number is 201608-14-2 and the empirical formula is C30H34F3N5O13.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies this product with an assay of ≥90% (HPLC). It is supplied for research purposes and is not intended for human or animal use, nor for pharmaceutical, food, or cosmetic applications. Tech Serve Solutions does not represent this product as USP, BP, EP, or other pharmacopoeia grade.
What are the safety and handling precautions for this chemical?
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This compound requires appropriate personal protective equipment, including eyeshields, gloves, and a type N95 (US) or type P1 (EN143) respirator filter. It is classified with a WGK Germany of 3, indicating a high hazard to water.
How is N-Acetyl-Asp-Glu-Val-Asp-7-amido-4-trifluoromethylcoumarin typically stored and shipped?
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The product should be stored at -20°C. TSS exports fine chemicals, pharmaceutical intermediates, and laboratory reagents globally.
How can I request a sample or obtain a quote?
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Please contact our customer service team to request a sample or obtain a quotation for N-Acetyl-Asp-Glu-Val-Asp-7-amido-4-trifluoromethylcoumarin.
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