Methylene Blue solution
Methylene Blue solution (CAS: 61-73-4, Formula: C16H18ClN3S) is a versatile dye with a molecular weight of 319.85 g/mol. This thiazine dye is primarily utilised as a biological stain in microscopy, histology, and microbiology. It is also employed in cell biology for various staining procedures, aiding in the visualisation of cellular structures and components. Its applications extend to specific diagnostic and research protocols.
- IUPAC
- Ehrlich’s reagent III,Loeffler’s Methylene Blue

Biological Staining
Methylene Blue solution is extensively used as a vital stain in microscopy for visualising cell nuclei, bacteria, and other microorganisms. It is particularly effective for simple staining of bacteria and capsule staining.
Histology and Hematology
In histological and hematological preparations, this dye aids in differentiating cellular components and can be used in staining techniques for blood smears. It is a common reagent in laboratories for routine tissue analysis.
Cell Biology Research
Researchers in cell biology utilise Methylene Blue solution for various staining protocols to observe cellular morphology and processes. Its use extends to applications within microfluidics and cellular imaging.
Microbiology Stains
This solution serves as a key reagent for simple stains of bacteria and in specific diagnostic tests within microbiology. It is also associated with staining solutions for capsule staining.
| Molecular weight | 319.85 |
|---|---|
| Empirical formula | C16H18ClN3S |
| Grade | for microbiology |
| Protective equipment | Eyeshields, Gloves |
|---|---|
| Water hazard class (WGK, DE) | 2 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Shelf Life | (limited shelf life, expiry date on the label) |
|---|
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is Methylene Blue solution used for?
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Methylene Blue solution is primarily used as a biological stain in microscopy, histology, and microbiology for visualising cellular structures, bacteria, and other microorganisms. It is also employed in cell biology research and specific diagnostic staining procedures.
What is the CAS number and chemical formula for Methylene Blue solution?
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The CAS number for Methylene Blue solution is 61-73-4, and its chemical formula is C16H18ClN3S.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies Methylene Blue solution for microbiology. For specific purity details and assays, please refer to the product's Certificate of Analysis or contact our sales team.
How should Methylene Blue solution be handled safely?
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Handle Methylene Blue solution with appropriate personal protective equipment, including eyeshields and gloves, as it can cause skin and eye irritation. Consult the Safety Data Sheet (SDS) for comprehensive handling and storage information. The WGK (Germany) is 2.
How is Methylene Blue solution packed and shipped?
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Methylene Blue solution is carefully packed according to international shipping regulations to ensure safe transit. TSS exports globally; please contact us for specific shipping and delivery information to your region.
How can I request a sample or quote for Methylene Blue solution?
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To request a sample or a quotation for Methylene Blue solution, please visit our website or contact our dedicated sales department with your specific requirements.
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