Methylene Violet 3RAX
Methylene Violet 3RAX (CAS: 4569-86-2), with the molecular formula C22H23ClN4 and a molecular weight of 378.90, is a vital dye used in biological and histological staining. It is recognised for its distinct absorption spectrum, with a lambda max at 557 nm. This compound serves as a key reagent in laboratory settings for visualising cellular structures and tissues, contributing to advancements in scientific research and diagnostics.
- IUPAC
- 3-Amino-7-(diethylamino)-5-phenyl phenazinium chloride,3-Amino-7-(diethylamino)-5-phenylphenazinium chloride,N,N-Diethylphenosafranine
- Synonyms
- 3-Amino-7-(diethylamino)-5-phenyl phenazinium chloride3-Amino-7-(diethylamino)-5-phenylphenazinium chlorideN,N-Diethylphenosafranine

Biological Staining
Methylene Violet 3RAX is employed as a stain in cell biology to visualise cellular components and structures. Its specific spectral properties aid in differentiating cellular elements under microscopic examination.
Histological Applications
In histology, this dye is used for staining tissue samples. It helps in the examination of tissue morphology, contributing to the diagnosis and study of biological tissues.
Research Reagent
As a laboratory reagent, Methylene Violet 3RAX supports various research protocols where accurate and reliable staining is essential. It is a valuable tool for scientific investigation.
| Molecular weight | 378.90 |
|---|---|
| Empirical formula | C22H23ClN4 |
| Melting point | 285 °C (dec.)(lit.) |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Colour Index Number | 50206 |
|---|---|
| Absorption | λmax 557 nm |
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is Methylene Violet 3RAX used for?
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Methylene Violet 3RAX is primarily used as a stain in cell biology and histology for visualising cellular structures and tissues, and as a general laboratory reagent.
What are the CAS number and formula for Methylene Violet 3RAX?
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The CAS number for Methylene Violet 3RAX is 4569-86-2, and its molecular formula is C22H23ClN4.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies Methylene Violet 3RAX with a Colour Index number of 50206. We do not represent this product as USP, BP, or EP grade.
How should Methylene Violet 3RAX be handled safely?
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Safe handling requires the use of eyeshields and gloves. A respirator filter of type N95 (US) or P1 (EN143) is recommended. Refer to the safety data sheet for comprehensive information. It is classified with WGK Germany 3.
Is Methylene Violet 3RAX a controlled substance?
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The provided data does not indicate that Methylene Violet 3RAX is a controlled substance.
How is Methylene Violet 3RAX packed, shipped, and exported?
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Tech Serve Solutions professionally packs, ships, and exports fine chemicals globally, adhering to all relevant regulations for safe transport.
How can I request a sample or quote for Methylene Violet 3RAX?
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Please contact our sales team through the website or by phone to request a sample or a quotation for Methylene Violet 3RAX.
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