Methylene blue
Methylene Blue trihydrate (CAS: 7220-79-3), with the molecular formula C16H18ClN3S · 3H2O and a molecular weight of 373.90, is a thiazine dye supplied by Tech Serve Solutions. This vibrant blue powder, certified by the Biological Stain Commission, is primarily used as a vital stain in biological and histological applications. It is also recognised for its utility in various in vitro diagnostic procedures and as a redox indicator.
- IUPAC
- 3,7-bis(Dimethylamino)phenazathionium chloride,Basic Blue 9,Tetramethylthionine chloride
- Synonyms
- 3,7-bis(Dimethylamino)phenazathionium chlorideBasic Blue 9Tetramethylthionine chloride
Biological Staining
Methylene blue is widely employed as a differential stain in microscopy for visualizing cell structures, nuclei, and bacteria. Its ability to bind with nucleic acids makes it invaluable in histology and hematology for tissue sample preparation and analysis.
In Vitro Diagnostics (IVD)
Certified for in vitro diagnostic use, this compound serves as a key reagent in various diagnostic assays and staining kits. It is employed to aid in the detection and characterization of biological samples.
Redox Indicator
The reversible reduction-oxidation properties of Methylene blue allow its use as a visual indicator in redox titrations and biochemical experiments. Its distinct colour change signals the endpoint of specific chemical reactions.
Research Reagent
As a core bioreagent and research essential, Methylene blue is utilized in life sciences for applications such as DNA/RNA electrophoresis and various laboratory investigations requiring staining or redox activity.
| Molecular weight | 373.90 |
|---|---|
| Empirical formula | C16H18ClN3S · 3H2O |
| Grade | certified by the Biological Stain Commission |
| Form | powder |
| Solubility | H2O: soluble4mg/4ml |
| Concentration | 1.4 % (w/v) in 95% ethanol |
| Composition | Dye content, ≥82% |
Hazard statements
- H302Harmful if swallowed
- H315Causes skin irritation
- H319Causes serious eye irritation
- H335May cause respiratory irritation
Precautionary statements
- P261Avoid breathing dust, fume, gas or vapours
- P305IF IN EYES
| Protective equipment | dust mask type N95 (US), Eyeshields, Gloves |
|---|---|
| Water hazard class (WGK, DE) | 3 |
| Hazard codes (EU) | Xn |
| Risk statements (R) | 22-36/37/38 |
| Safety statements (S) | 26-36 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Colour Index Number | 52015 |
|---|---|
| Gene Information | human ... CYP1A2(1544), GSR(2936) |
| Ivd | for in vitro diagnostic use |
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is Methylene Blue used for?
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Methylene blue is primarily used as a biological stain for microscopy, in histology and hematology, as a reagent in in vitro diagnostic (IVD) applications, and as a redox indicator in laboratory research. It is also noted for its utility in DNA/RNA electrophoresis.
What are the CAS number and formula for Methylene Blue?
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The CAS number for Methylene Blue trihydrate is 7220-79-3, and its empirical formula is C16H18ClN3S · 3H2O.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies Methylene Blue certified by the Biological Stain Commission with a dye content of ≥82%. It is supplied as a powder. This product is intended for laboratory use and is not represented as USP, BP, or EP grade.
What are the safety and handling precautions for Methylene Blue?
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Methylene Blue is classified with GHS07 symbol and a 'Warning' signal word, presenting hazards such as H302 (harmful if swallowed), H315 (causes skin irritation), H319 (causes serious eye irritation), and H335 (may cause respiratory irritation). Appropriate personal protective equipment, including a dust mask type N95, eyeshields, and gloves, should be used. Handle in a well-ventilated area.
How is Methylene Blue packed, shipped, and exported?
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Methylene Blue is carefully packed in appropriate containers to ensure integrity during transit. As a global supplier and exporter, Tech Serve Solutions adheres to all relevant shipping and export regulations for chemical products.
How can I request a sample or quote for Methylene Blue?
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To request a sample or a quote for Methylene Blue, please contact our sales team through the website or by phone. Provide your specific requirements, and we will respond promptly.
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