Methyl violet 2B
Methyl violet 2B (CAS: 8004-87-3) is a violet triphenylmethane dye with a specified dye content of 75%. It exhibits an absorption maximum (λmax) at 584 nm. This versatile stain is widely utilised in biological staining techniques, particularly within cell biology, hematology, and histology. Its certified status by the Biological Stain Commission underscores its reliability for diagnostic and research applications. Methyl violet 2B is a key reagent for visualising cellular structures.
- IUPAC
- Basic Violet 1
- Synonyms
- Basic Violet 1Gentian Violet 2BCrystal Violet 2BPyoktanin BlueAniline Violet 2B

Biological Staining
As a certified stain, Methyl Violet 2B is frequently employed in histology and hematology for the differential staining of cells and tissues. Its distinct colour allows for clear visualisation of cellular components and microorganisms in microscopy.
Cell Biology Research
In cell biology, this dye serves as a vital tool for observing nuclear and cytoplasmic structures. It aids researchers in distinguishing between different cell types and analysing cellular morphology.
Microscopy Reagent
Methyl violet 2B is a common laboratory reagent used in various microscopy applications. It provides contrast and aids in the identification of stained elements under microscopic examination.
| Grade | certified by the Biological Stain Commission |
|---|---|
| Composition | Dye content, 75% |
| Melting point | 137 °C (dec.)(lit.) |


Hazard statements
- H302Harmful if swallowed
- H400Very toxic to aquatic life
Precautionary statements
- P273Avoid release to the environment
| Protective equipment | dust mask type N95 (US), Eyeshields, Faceshields, Gloves |
|---|---|
| Transport (UN / ADR) | UN 3077 9 / PGIII |
| Water hazard class (WGK, DE) | 3 |
| Hazard codes (EU) | Xn,N |
| Risk statements (R) | 22-50 |
| Safety statements (S) | 61 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Colour Index Number | 42535 |
|---|---|
| Absorption | λmax 584 nm |
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is Methyl Violet 2B used for?
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Methyl Violet 2B is primarily used as a biological stain in cell biology, hematology, and histology for visualising cellular structures and microorganisms.
What are the CAS number and formula for Methyl Violet 2B?
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The CAS number for Methyl Violet 2B is 8004-87-3. Its chemical formula is not specified in the provided data.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies Methyl Violet 2B as a certified stain by the Biological Stain Commission, with a specified dye content of 75%.
What are the primary safety hazards associated with Methyl Violet 2B?
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Methyl Violet 2B is classified with GHS symbols GHS07 and GHS09, carrying a "Warning" signal word. It has hazard statements H302 (Harmful if swallowed) and H400 (Very toxic to aquatic life). Appropriate personal protective equipment, including a dust mask (type N95), eyeshields, face shields, and gloves, should be used.
How is Methyl Violet 2B packaged and shipped?
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Methyl Violet 2B is shipped under UN 3077 classification, Hazard Class 9, Packing Group III, indicating it is an environment-hazardous substance, solid, n.o.s.
How can I request a sample or quote for Methyl Violet 2B?
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To request a sample or a quote for Methyl Violet 2B, please contact Tech Serve Solutions directly through our website or by phone.
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