L-Leucine-7-amido-4-methylcoumarin hydrochloride
L-Leucine-7-amido-4-methylcoumarin hydrochloride (CAS 62480-44-8), with molecular formula C16H20N2O3 · HCl and a molecular weight of 324.80 g/mol, is a fluorescent enzyme substrate. This compound is primarily utilised in biochemical research for the detection and quantification of enzyme activity, particularly proteases. Its specific fluorescence properties upon cleavage make it a valuable tool in cell biology and enzyme kinetics studies.
Protease Activity Assays
L-Leucine-7-amido-4-methylcoumarin hydrochloride serves as a sensitive substrate for assays measuring the activity of various proteases. Upon enzymatic cleavage, it releases a fluorescent moiety, allowing for real-time monitoring of enzyme kinetics.
Cell Biology Research
This compound is employed in cell biology to investigate intracellular enzyme processes and signaling pathways. Its fluorescence can be used to visualise enzyme localisation and activity within cellular environments.
Enzyme Characterisation
It is a useful reagent for the characterisation of newly discovered proteases or for studying known enzymes in complex biological matrices. The reliable fluorescence signal aids in determining enzyme specificity and catalytic efficiency.
| Molecular weight | 324.80 |
|---|---|
| Empirical formula | C16H20N2O3 · HCl |
| Storage temperature | −20°C |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Fluorescence | λex 327 nm; λem 349 nm (pH 8.0)λex 380 nm; λem 440 nm (Reaction product) |
|---|
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is L-Leucine-7-amido-4-methylcoumarin hydrochloride used for?
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L-Leucine-7-amido-4-methylcoumarin hydrochloride is used as a fluorescent enzyme substrate, primarily for the detection and quantification of protease activity in biochemical and cell biology research.
What is the CAS number and formula for L-Leucine-7-amido-4-methylcoumarin hydrochloride?
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The CAS number is 62480-44-8, and the molecular formula is C16H20N2O3 · HCl.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies this product for research and laboratory use. The specific grade and purity are detailed in the product's Certificate of Analysis, and TSS does not represent it as USP, BP, EP, or other pharmacopoeial grades unless explicitly stated.
How should L-Leucine-7-amido-4-methylcoumarin hydrochloride be handled?
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Handle with appropriate personal protective equipment, including eyeshields and gloves. A respirator filter of type N95 (US) or P1 (EN143) is recommended. Refer to the Safety Data Sheet for detailed handling and hazard information. The substance has a WGK Germany rating of 3.
Is this product available for export?
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Yes, Tech Serve Solutions is a global supplier and exporter of fine chemicals, and this product can be shipped internationally. Please contact us for export details and availability.
How can I request a sample or quote?
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To request a sample or a quotation for L-Leucine-7-amido-4-methylcoumarin hydrochloride, please visit the product page on our website and use the designated request forms, or contact our sales team directly.
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