L-Leucine-p-nitroanilide
L-Leucine-p-nitroanilide (CAS: 4178-93-2, C12H17N3O3) is a biochemical reagent with a molecular weight of 251.28 g/mol. It serves as a chromogenic substrate, primarily used in the enzymatic assay of proteases, particularly leucine aminopeptidases. This compound is instrumental in biochemical research for studying enzyme kinetics and activity, offering a reliable method for quantifying enzyme levels in biological samples.
- IUPAC
- L-Leucine-4-nitroanilide
- Synonyms
- L-Leucine-4-nitroanilide
Enzyme Substrate for Protease Assays
L-Leucine-p-nitroanilide is widely employed as a substrate for the spectrophotometric determination of leucine aminopeptidase activity. Upon enzymatic cleavage, it releases p-nitroaniline, which can be quantified coloremetrically.
Biochemical Research
This compound is a valuable tool in biochemical research for understanding enzyme mechanisms, kinetics, and inhibition studies. Its use allows for precise measurement of enzyme activity in various biological contexts.
Cell Biology and Signaling Studies
As a substrate for enzymes involved in cellular processes, L-Leucine-p-nitroanilide finds application in studies related to cell biology and signaling pathways where proteases play a critical role.
| Molecular weight | 251.28 |
|---|---|
| Empirical formula | C12H17N3O3 |
| Storage temperature | 2-8°C |
Hazard statements
- H317May cause an allergic skin reaction
- H319Causes serious eye irritation
Precautionary statements
- P280Wear protective gloves, clothing and eye/face protection
- P305IF IN EYES
| Protective equipment | dust mask type N95 (US), Eyeshields, Faceshields, Gloves |
|---|---|
| Water hazard class (WGK, DE) | 3 |
| Hazard codes (EU) | Xi |
| Risk statements (R) | 36-43 |
| Safety statements (S) | 26-36/37 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is L-Leucine-p-nitroanilide used for?
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L-Leucine-p-nitroanilide is primarily used as a chromogenic substrate in enzymatic assays, particularly for quantifying the activity of leucine aminopeptidase and other proteases. It releases p-nitroaniline upon enzymatic cleavage, allowing for spectrophotometric measurement.
What are the CAS number and formula for L-Leucine-p-nitroanilide?
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The CAS number for L-Leucine-p-nitroanilide is 4178-93-2, and its molecular formula is C12H17N3O3.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies L-Leucine-p-nitroanilide for research purposes. The specific grade and purity are detailed in the product specifications. TSS does not represent this product as USP, BP, or EP grade.
What are the safety and handling precautions for L-Leucine-p-nitroanilide?
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L-Leucine-p-nitroanilide is classified with a Warning signal word, with hazards including skin sensitisation (H317) and serious eye irritation (H319). Appropriate personal protective equipment, including a dust mask (type N95 US), eyeshields, face shields, and gloves, should be worn. Ensure good ventilation and avoid dust formation.
How is L-Leucine-p-nitroanilide packed and shipped?
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L-Leucine-p-nitroanilide is typically stored at 2-8°C. For export and shipping, it is packaged according to international regulations for chemical substances to ensure safe transit.
How can I request a sample or quote for L-Leucine-p-nitroanilide?
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To request a sample or a quotation for L-Leucine-p-nitroanilide, please contact our sales department via the website or direct email, providing your required quantity and shipping details.
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