L-Leucine p-nitroanilide hydrochloride
L-Leucine p-nitroanilide hydrochloride (CAS: 16010-98-3; Formula: C12H17N3O3 · HCl) is a valuable biochemical reagent. It serves as a chromogenic substrate for the determination of enzyme activity, particularly peptidases and proteases. Its structure allows for the release of a coloured p-nitroaniline moiety upon enzymatic cleavage, facilitating quantitative analysis in biochemical research and diagnostics. The compound is supplied by Tech Serve Solutions with a minimum assay of 98% by TLC.
Enzyme Activity Assays
Used as a chromogenic substrate for the detection and quantification of peptidases and proteases. Enzymatic hydrolysis releases p-nitroaniline, allowing for spectrophotometric measurement of enzyme kinetics.
Biochemical Research
A versatile tool in biochemical laboratories for studying enzyme mechanisms and screening for enzyme inhibitors. Its specific cleavage properties make it suitable for targeted research applications.
Diagnostic Reagent Development
Contributes to the development of diagnostic kits where enzyme activity is a key biomarker. Enables rapid and sensitive detection of relevant enzymes in biological samples.
| Molecular weight | 287.74 |
|---|---|
| Empirical formula | C12H17N3O3 · HCl |
| Assay | ≥98% (TLC) |
| Storage temperature | 2-8°C |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is L-Leucine p-nitroanilide hydrochloride used for?
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L-Leucine p-nitroanilide hydrochloride is primarily used as a chromogenic substrate for the assay of enzyme activity, particularly peptidases and proteases, in biochemical research and diagnostic applications.
What is the CAS number and chemical formula?
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The CAS number is 16010-98-3 and the chemical formula is C12H17N3O3 · HCl.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies L-Leucine p-nitroanilide hydrochloride with a minimum assay of 98% (determined by TLC). We do not represent this product as USP, BP, EP, or pharmacopoeia grade.
How should L-Leucine p-nitroanilide hydrochloride be handled and stored?
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Handling requires appropriate personal protective equipment, including eyeshields, gloves, and a P1 (EN143) or N95 (US) respirator filter. Store at 2-8°C.
Is this compound classified as hazardous?
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Yes, this compound has a WGK rating of 3 in Germany, indicating a high hazard to water. Appropriate safety precautions must be observed during handling and disposal.
How can I request a sample or quote?
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To request a sample or a quote, please contact our sales team through the website or by phone, providing the product name and CAS number.
Is L-Leucine p-nitroanilide hydrochloride shipped internationally?
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Yes, Tech Serve Solutions is a global supplier and exporter of fine chemicals and reagents, including L-Leucine p-nitroanilide hydrochloride.
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