Hygromycin B from Streptomyces hygroscopicus

CAS 31282-04-9C20H37N3O13Biochemicals & Reagents

Hygromycin B (CAS 31282-04-9, C20H37N3O13) is an aminoglycoside antibiotic derived from *Streptomyces hygroscopicus*. This potent compound, with a molecular weight of 527.52, is primarily utilised as a selection agent in molecular biology and genetic research for transformed cells. It functions by interfering with protein synthesis, offering effective selection at specific concentrations for prokaryotic, lower eukaryotic, and higher eukaryotic systems.

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Hygromycin B from Streptomyces hygroscopicus (C20H37N3O13) — chemical structure, CAS 31282-04-9; Life Science, fine chemical supplied by Tech Serve Solutions

AminoglycosidesAntibioticsAntibiotics A to ZAntibiotics G-MAntibiotics and AntimycoticsAntibiotics by ApplicationAntifungalBiochemicals and ReagentsChemical Structure ClassGenetic Marker Selection

01 /Applications

Genetic Marker Selection

Hygromycin B is employed as a selection agent to identify and isolate cells that have successfully integrated the hygromycin resistance gene. It is recommended for use at concentrations ranging from 100-800 μg/mL, with specific optimal ranges for different organisms.

Protein Synthesis Research

This antibiotic aids in studying the mechanisms of protein synthesis, specifically focusing on the translocation step at the 70S ribosome and misreading of the mRNA template. Its inhibitory action provides a tool for biochemical and molecular biology investigations.

Antiviral Agent Research

Hygromycin B has demonstrated utility as an antiviral agent. Its ability to selectively penetrate virus-infected cells, which exhibit increased permeability, allows for the inhibition of viral translation processes.

02 /Properties

Molecular weight	527.52
Empirical formula	C20H37N3O13
Assay	≥60% (HPLC)
Form	lyophilized powder
Concentration	45-60 mg/mL in H2O
Storage temperature	2-8°C
Application	Hygromycin B is an aminoglycoside antibiotic isolated from Streptomyces hygroscopicus. It has been used to study protein synthesis at the level of the 70S ribosome translocation and mRNA template misreading, as an antiviral agent by selectively penetrating cells rendered permeable by virus infection and inhibiting translation, and as a selection agent for hygromycin resistance gene transformed cells. It is recommended for use as a selection agent at 100-800 μg/mL, specifically at 100 μg/mL for prokaryotes, 200 μg/mL for lower eukaryotes and 150-400 μg/mL for higher eukaryotes.
Suitability	plant cell culture tested

03 /Safety & handling

GHS hazard pictogram: Corrosive (GHS05) — Corrosive

GHS hazard pictogram: Toxic (GHS06) — Toxic

GHS hazard pictogram: Health hazard (GHS08) — Health hazard

Danger

Hazard statements

H300Fatal if swallowed
H318Causes serious eye damage
H334May cause allergy or asthma symptoms if inhaled

Precautionary statements

P260Do not breathe dust, fume, gas, mist, vapours or spray
P264Wash thoroughly after handling
P280Wear protective gloves, clothing and eye/face protection
P284Wear respiratory protection
P301IF SWALLOWED
P302IF ON SKIN

Transport (UN / ADR)	UN 3462 6.1 / PGI
Water hazard class (WGK, DE)	3
Hazard codes (EU)	T+
Risk statements (R)	26/27/28-41-42/43
Safety statements (S)	22-26-28-36/37/39-45

Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.

04 /Identifiers & registry

CAS number

31282-04-9

MDL number

MFCD06795479

05 /Additional specifications

Product Line	BioReagent
Market Segment	Agriculture
Purified By	ion-exchange chromatography
Sterility	γ-irradiated

06 /Quality & supply

Documentation

Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.

Supply & logistics

Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.

07 /Frequently asked questions

What is Hygromycin B used for?

Hygromycin B is primarily used as a selection agent in molecular biology for transformed cells, for studying protein synthesis, and in research as an antiviral agent.

What is the CAS number and formula for Hygromycin B?

The CAS number is 31282-04-9 and the empirical formula is C20H37N3O13.

What grade and purity does Tech Serve Solutions supply?

Tech Serve Solutions supplies Hygromycin B with an assay of ≥60% (HPLC). This product is plant cell culture tested. We do not represent this product as USP, BP, or EP grade.

How should Hygromycin B be handled safely?

Hygromycin B is a dangerous substance. It is toxic if swallowed, in contact with skin, or if inhaled, and causes serious eye damage. It may also cause allergy or asthma symptoms or breathing difficulties if inhaled. Handle with extreme caution in a well-ventilated area, wearing appropriate personal protective equipment including gloves, eye protection, and respiratory protection. Refer to the Safety Data Sheet for full handling instructions.

Is Hygromycin B packed and shipped by TSS?

Yes, Tech Serve Solutions is a specialist global supplier and exporter of fine chemicals, including Hygromycin B, which is carefully packed and exported worldwide.