Fluorescein isothiocyanate isomer I
Fluorescein isothiocyanate isomer I (CAS: 3326-32-7; C21H11NO5S) is a vital biochemical reagent with a molecular weight of 389.38 g/mol. This yellow to orange powder is primarily used for conjugating fluorescent labels to biomolecules, particularly proteins, through reactions with primary amines. Its distinct fluorescent properties, with excitation and emission maxima at 492 nm and 518 nm respectively, make it invaluable for various biochemical and cell biology applications, including microsequencing.
- IUPAC
- FITC,Fluorescein 5-isothiocyanate
- Synonyms
- FITC,Isomer I,Fluorescein 5-isothiocyanate
Protein Labeling
Fluorescein isothiocyanate isomer I serves as a key reagent for covalently labeling proteins. It reacts with free amino groups on proteins, imparting a fluorescent tag that can be detected using fluorescence microscopy and other sensitive detection methods.
Biomolecular Conjugation
This compound is widely employed for conjugating fluorescent markers to various biomolecules, enabling their visualization and tracking in biological systems. It is suitable for labeling antibodies, peptides, and nucleic acids.
Microsequencing
Fluorescein isothiocyanate isomer I is utilised in the microsequencing of proteins and peptides. Its application in conjunction with High-Performance Liquid Chromatography (HPLC) aids in the precise determination of amino acid sequences.
| Molecular weight | 389.38 |
|---|---|
| Empirical formula | C21H11NO5S |
| Assay | 90% |
| Form | powder |
| Solubility | acetone: soluble1 mg/mL |
| Storage temperature | 2-8°C |
| Application | Reagent for the FITC labeling of proteins; Microsequencing of proteins and peptides (HPLC) |
| Melting point | >360 °C(lit.) |
| Suitability | suitable for protein labeling |
Hazard statements
- H317May cause an allergic skin reaction
- H334May cause allergy or asthma symptoms if inhaled
Precautionary statements
- P261Avoid breathing dust, fume, gas or vapours
- P280Wear protective gloves, clothing and eye/face protection
- P342
| Protective equipment | dust mask type N95 (US), Eyeshields, Faceshields, Gloves |
|---|---|
| Water hazard class (WGK, DE) | 3 |
| Hazard codes (EU) | Xn |
| Risk statements (R) | 42/43 |
| Safety statements (S) | 22-36/37-45 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Absorption | λmax 499 nm |
|---|---|
| Fluorescence | λex 492 nm; λem 518 nm (green) |
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is Fluorescein isothiocyanate isomer I used for?
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Fluorescein isothiocyanate isomer I is primarily used as a reagent for labeling proteins with a fluorescent tag, and in the microsequencing of proteins and peptides via HPLC. It is suitable for protein labeling.
What are the CAS number and molecular formula of Fluorescein isothiocyanate isomer I?
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The CAS number for Fluorescein isothiocyanate isomer I is 3326-32-7, and its molecular formula is C21H11NO5S.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies Fluorescein isothiocyanate isomer I with an assay of 90%. We do not represent this product as USP, BP, or EP grade; it is supplied for research and laboratory use.
How should Fluorescein isothiocyanate isomer I be handled and stored safely?
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This product may cause an allergic skin reaction (H317) and allergic respiratory reaction (H334). Wear appropriate personal protective equipment, including a dust mask (type N95), eyeshields, faceshields, and gloves. Store at 2-8°C.
Is Fluorescein isothiocyanate isomer I supplied internationally by TSS?
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Yes, Tech Serve Solutions is a specialist global supplier and exporter of fine chemicals, including Fluorescein isothiocyanate isomer I.
How can I request a sample or quote for Fluorescein isothiocyanate isomer I?
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Please contact our customer service team via our website or by phone to request a sample or a quotation for Fluorescein isothiocyanate isomer I.
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