Fluorescein diacetate 6-isothiocyanate
Fluorescein diacetate 6-isothiocyanate (CAS: 890090-49-0; Formula: C25H15NO7S; MW: 473.45) is a specialized biochemical reagent. As a structural analogue of fluorescein, it reacts with secondary amines, serving as a haptenic probe in various research applications. Its fluorescent properties, with excitation and emission maxima at 496 nm and 517 nm respectively, make it valuable for labelling and detection in biological systems.
- IUPAC
- Diacetyl-6-FITC,Fluorescein-6-isothiocyanate diacetate

Fluorescent Haptenic Probe
Fluorescein diacetate 6-isothiocyanate is utilized as a fluorescent probe due to its structural similarity to fluorescein. It is specifically employed to react with secondary amines, functioning as a haptenic label in biochemical assays.
Biochemical Research
This compound serves as a valuable reagent in biochemical research, particularly in studies involving fluorescent labelling. Its capacity to conjugate with amines allows for the detection and tracking of biological molecules.
Diagnostic Assays
As a fluorescent label, Fluorescein diacetate 6-isothiocyanate can be incorporated into various diagnostic assays where fluorescent detection is required. Its fluorescence emission at 517 nm aids in signal generation for sensitive detection.
| Molecular weight | 473.45 |
|---|---|
| Empirical formula | C25H15NO7S |
| Assay | ≥90% (HPLC) |
| Application | Fluorescein diacetate 6-isothiocyanate (diacetyl-6-FITC, F6ITC), a structural analogue of fluorescein reactive with secondary amines, is used as an isomeric haptenic probe. |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Fluorescence | λex 496 nm; λem 517 nm in 0.1 M Tris pH 8.0, esterase |
|---|
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is Fluorescein diacetate 6-isothiocyanate used for?
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Fluorescein diacetate 6-isothiocyanate is used as an isomeric haptenic probe, reacting with secondary amines for fluorescent labelling in biochemical research and diagnostic assays.
What is the CAS number and formula for Fluorescein diacetate 6-isothiocyanate?
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The CAS number is 890090-49-0 and the empirical formula (Hill notation) is C25H15NO7S.
What grade and purity does Tech Serve Solutions supply for Fluorescein diacetate 6-isothiocyanate?
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Tech Serve Solutions supplies Fluorescein diacetate 6-isothiocyanate with a purity of ≥90% (HPLC). TSS does not represent this product as USP, BP, or EP grade.
What safety precautions should be taken when handling Fluorescein diacetate 6-isothiocyanate?
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When handling Fluorescein diacetate 6-isothiocyanate, it is essential to use safety measures including eyeshields and gloves. A type N95 (US) or type P1 (EN143) respirator filter should be used if necessary. Refer to the safety data sheet for full guidance.
How is Fluorescein diacetate 6-isothiocyanate packed and shipped?
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Fluorescein diacetate 6-isothiocyanate is carefully packed for safe transport and export by Tech Serve Solutions, adhering to international shipping regulations for chemicals.
How can I request a quote or sample for Fluorescein diacetate 6-isothiocyanate?
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To request a quote or a sample of Fluorescein diacetate 6-isothiocyanate, please contact the Tech Serve Solutions sales team through our website or by phone.
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