Fluorescein diacetate 5-maleimide
Fluorescein diacetate 5-maleimide (CAS: 150322-01-3; C28H17NO9; MW: 511.44) is a fluorescent labelling reagent, widely used for its ability to couple with thiols. This compound is suitable for fluorescence applications, emitting at λex 492 nm and λem 530 nm after derivatization and esterase cleavage. Its solubility in DMF, DMSO, and dioxane makes it versatile for various biochemical and analytical research purposes.
- IUPAC
- 5-Maleimido-fluorescein diacetate,Fluorescein-5-maleimide diacetate
Fluorescent Labeling
Utilised for attaching fluorescent tags to biomolecules. Its maleimide group selectively reacts with thiol groups, enabling precise labelling for detection and imaging.
Thiol Detection
Serves as a probe for detecting the presence of thiol (-SH) groups in biological samples. The resulting fluorescence allows for quantitative analysis.
Enzyme Activity Assays
The diacetate ester linkage can be cleaved by esterases, releasing a fluorescent signal. This property is leveraged in assays to measure esterase activity.
Flow Cytometry Reagent
Applicable in flow cytometry for cell surface or intracellular labelling. It allows for the analysis of cellular populations based on fluorescence intensity.
| Molecular weight | 511.44 |
|---|---|
| Empirical formula | C28H17NO9 |
| Solubility | DMF: solubleDMSO: solubledioxane: soluble |
| Storage temperature | −20°C |
| Suitability | corresponds for coupling to thiolssuitable for fluorescence |
Hazard statements
- H315Causes skin irritation
- H319Causes serious eye irritation
- H335May cause respiratory irritation
Precautionary statements
- P261Avoid breathing dust, fume, gas or vapours
- P305IF IN EYES
| Protective equipment | dust mask type N95 (US), Eyeshields, Gloves |
|---|---|
| Water hazard class (WGK, DE) | 3 |
| Hazard codes (EU) | Xi |
| Risk statements (R) | 36/37/38 |
| Safety statements (S) | 26-36 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Fluorescence | λex 492 nm; λem 530 nm in 0.1 M phosphate pH 7.0 (after derivatization with 2-mercaptoethanol and after cleavage by esterase) |
|---|
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is Fluorescein diacetate 5-maleimide used for?
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Fluorescein diacetate 5-maleimide is primarily used for fluorescent labelling of thiol groups in biomolecules and for assays measuring esterase activity due to its reactive maleimide moiety and cleavable diacetate ester.
What is the CAS number and formula for Fluorescein diacetate 5-maleimide?
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The CAS number is 150322-01-3 and the empirical formula is C28H17NO9.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies Fluorescein diacetate 5-maleimide for research purposes. The specific purity/assay is available on the product's Certificate of Analysis. TSS does not represent this product as USP, BP, EP, or any other pharmacopoeia grade.
How should Fluorescein diacetate 5-maleimide be handled safely?
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Handle Fluorescein diacetate 5-maleimide with appropriate personal protective equipment, including a dust mask (type N95 or equivalent), eyeshields, and gloves, to avoid skin, eye, and respiratory irritation (H315-H319-H335).
How is Fluorescein diacetate 5-maleimide packed, shipped, and exported?
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Fluorescein diacetate 5-maleimide is typically packed according to standard chemical safety protocols for reagents, stored at -20°C, and exported globally by Tech Serve Solutions.
How can I request a sample or quote for Fluorescein diacetate 5-maleimide?
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To request a sample or quote, please contact our sales team through the Tech Serve Solutions website or by calling us directly.
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