Eosin Y
Eosin Y (CAS: 15086-94-9), a tetrabrominated derivative of fluorescein, presents as a vibrant red crystalline powder. With the molecular formula C20H8Br4O5 and a molecular weight of 647.89 g/mol, it is an important acidic dye widely employed in histology and cell biology for differential staining. Its vivid colour and staining properties make it indispensable for visualizing cellular structures in microscopic preparations.
- IUPAC
- 2′,4′,5′,7′-Tetrabromofluorescein,Acid Red 87,Bromo acid J. TS, XL, or XX,Bromofluorescein,Bronze Bromo ES,Eosin yellowish,Solvent red 43
- Synonyms
- 2′,4′,5′,7′-TetrabromofluoresceinAcid Red 87Bromo acid J. TS, XL, or XXBromofluoresceinBronze Bromo ESEosin yellowishSolvent red 43

Histology Staining
Eosin Y is a fundamental counterstain in H&E (Haematoxylin and Eosin) staining, providing bright pink to red coloration to cytoplasm, collagen, and other extracellular materials. This aids in the differentiation of tissue components.
Cytology Applications
In cytological preparations, Eosin Y is used to stain various cellular elements, enhancing contrast and aiding in the identification of cellular morphology. It is particularly useful for routine screening of smears.
Biological Stains
As part of the 'E-F' category of stains, Eosin Y functions as an acidic dye, binding to basic components of cells and tissues, such as positively charged proteins in the cytoplasm.
| Molecular weight | 647.89 |
|---|---|
| Empirical formula | C20H8Br4O5 |
| Solubility | ethanol: soluble1 mg/mL |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Colour Index Number | 45380:2 |
|---|
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is Eosin Y used for?
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Eosin Y is primarily used as a counterstain in histology and cytology for visualizing cellular structures and tissue components under microscopy, particularly in H&E staining protocols.
What are the CAS number and chemical formula for Eosin Y?
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The CAS number for Eosin Y is 15086-94-9, and its empirical formula is C20H8Br4O5.
What grade and purity does Tech Serve Solutions supply for Eosin Y?
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Tech Serve Solutions supplies Eosin Y with a Colour Index Number of 45380:2. We do not represent this product as USP, BP, EP, or any pharmacopoeia grade; it is suitable for research and technical applications.
What are the safety precautions when handling Eosin Y?
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When handling Eosin Y, it is essential to wear eyeshields and gloves. A type N95 (US) or type P1 (EN143) respirator filter should be used if airborne dust is generated. Handle with appropriate personal protective equipment and in a well-ventilated area.
How is Eosin Y packed and shipped?
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Eosin Y is carefully packed to ensure safe transit and is exported globally by Tech Serve Solutions, adhering to all relevant shipping regulations for chemical substances.
How can I request a quote or sample for Eosin Y?
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To request a quote or a sample of Eosin Y, please contact our sales team through the website or by phone, providing your specific requirements.
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