Eosin B
Eosin B (CAS 548-24-3) is a bromine-containing xanthene dye with the molecular formula C₂₀H₆N₂O₉Br₂Na₂ and a molecular weight of 624.06 g/mol. It is a water-soluble, red fluorescent compound primarily employed as a biological stain in histology and hematology. Eosin B is valued for its ability to differentially stain cellular components, aiding in microscopic examination and diagnosis.
- IUPAC
- 4′,5′-Dibromo-2′,7′-dinitrofluorescein disodium salt,Acid Red 91,Eosin Scarlet,Eosin bluish,Saffrosine
- Synonyms
- 4′,5′-Dibromo-2′,7′-dinitrofluorescein disodium saltAcid Red 91Eosin ScarletEosin bluishSaffrosine
Histological Staining
Eosin B is widely used as a counterstain in hematoxylin and eosin (H&E) staining protocols. It effectively stains cytoplasm, connective tissue, and other extracellular elements a pink to red hue, providing contrast to the blue nuclei stained by hematoxylin.
Cytological Staining
In cytology, Eosin B helps in the differential staining of cells. It is particularly useful for highlighting cellular morphology in various biological samples, aiding in the identification of normal and abnormal cells.
Microbiological Staining
This dye can be employed in certain microbiological staining techniques to visualize cellular structures within microorganisms, contributing to their identification and characterisation.
Fluorescence Microscopy
With its fluorescent properties, Eosin B can be utilized in specific fluorescence microscopy applications to stain and visualize cellular structures, offering enhanced imaging capabilities.
| Molecular weight | 624.06 |
|---|---|
| Linear formula | C20H6N2O9Br2Na2 |
| Grade | certified by the Biological Stain Commission |
| Composition | Dye content, 90% |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Colour Index Number | 45400 |
|---|---|
| Absorption | λmax 395 nmλmax 514 nm |
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is Eosin B used for?
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Eosin B is primarily used as a biological stain in histology and cytology for differentially staining cellular components in microscopic examination.
What are the CAS number and formula for Eosin B?
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The CAS number for Eosin B is 548-24-3, and its linear formula is C₂₀H₆N₂O₉Br₂Na₂.
What grade and purity does Tech Serve Solutions supply for Eosin B?
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Tech Serve Solutions supplies Eosin B certified by the Biological Stain Commission with a dye content of 90%.
How should Eosin B be handled safely?
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When handling Eosin B, it is essential to use personal protective equipment including eyeshields and gloves. A respirator filter of type N95 (US) or P1 (EN143) should be used. Eosin B is assigned a WGK Germany classification of 3, indicating it is hazardous to water.
Is Eosin B a controlled substance?
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The provided data does not indicate that Eosin B is a controlled substance.
How is Eosin B packed and shipped?
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Eosin B is packed and shipped by Tech Serve Solutions in accordance with international regulations for chemical exports. Please contact us for specific details.
How can I request a quote or sample for Eosin B?
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To request a quote or a sample of Eosin B, please contact our sales department through the website or by phone.
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