Eosin B
Eosin B (CAS: 56360-46-4), with the empirical formula C20H8Br2N2O9 and a molecular weight of 580.09 g/mol, is a synthetic organic dye. This reddish-brown powder is primarily utilised as a stain in biological and histological applications for differential staining of tissues and cells. Its distinct spectral absorption at 522 nm facilitates its use in microscopy.
- IUPAC
- Acid Red 91

Histological Staining
Eosin B is employed as a counterstain in histology and cytology, differentiating cellular structures, particularly the cytoplasm and connective tissues. It works effectively in conjunction with nuclear stains like haematoxylin.
Cell Biology Research
In cell biology, Eosin B serves as a vital stain for visualising cellular morphology and architecture under microscopy. It aids in identifying and characterising different cell types and subcellular components.
Differential Staining
This dye is part of differential staining techniques used in haematology and histology laboratories. It helps distinguish between different tissues and pathological changes, aiding in diagnosis.
| Molecular weight | 580.09 |
|---|---|
| Empirical formula | C20H8Br2N2O9 |
| Solubility | spirit: soluble |
| Melting point | 275 °C (dec.)(lit.) |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Colour Index Number | 45400 |
|---|---|
| Absorption | λmax 522 nm |
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is Eosin B used for?
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Eosin B is primarily used as a histological and cytological stain for differential staining of tissues and cells, particularly in cell biology and haematology.
What are the CAS number and formula for Eosin B?
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The CAS number for Eosin B is 56360-46-4, and its empirical formula is C20H8Br2N2O9.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies Eosin B as a technical/research grade chemical. We do not represent this product as USP, BP, EP, or any other pharmacopoeia grade.
How should Eosin B be handled safely?
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Handle Eosin B with appropriate personal protective equipment, including eyeshields and gloves. Use a type N95 (US) or type P1 (EN143) respirator filter if dust is generated. It has a WGK of 3 in Germany, indicating a high hazard.
Can Eosin B be exported?
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Yes, Tech Serve Solutions specialises in the global supply and export of fine chemicals, including Eosin B.
How can I request a sample or quote for Eosin B?
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Please contact our sales team via the website or direct email to request a sample or a formal quotation for Eosin B.
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