E-64

CAS 66701-25-5C15H27N5O5Biochemicals & Reagents

E-64 (CAS: 66701-25-5), with the molecular formula C15H27N5O5 and a molecular weight of 357.41 g/mol, is a potent inhibitor of cysteine proteases. It functions as an effective ligand for affinity purification of these enzymes. This compound is a valuable tool in biochemical research, particularly in studies involving enzyme mechanisms and protein purification, offering high specificity in its interactions.

IUPAC: trans-Epoxysuccinyl-L-leucylamido(4-guanidino)butane,L-trans-3-Carboxyoxiran-2-carbonyl-L-leucylagmatine,N-(trans-Epoxysuccinyl)-L-leucine 4-guanidinobutylamide
Synonyms: trans-Epoxysuccinyl-L-leucylamido(4-guanidino)butaneL-trans-3-Carboxyoxiran-2-carbonyl-L-leucylagmatineN-(trans-Epoxysuccinyl)-L-leucine 4-guanidinobutylamide

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E-64 (C15H27N5O5) — chemical structure, CAS 66701-25-5; Life Science, fine chemical supplied by Tech Serve Solutions

Bioactive Small MoleculesBiochemicals and ReagentsCell BiologyEnzyme InhibitorsEnzyme Inhibitors by TypeEnzymesInhibitorsand Substrates

01 /Applications

Cysteine Protease Affinity Purification

E-64 serves as an effective ligand for the affinity purification of cysteine proteases. When immobilised onto a suitable affinity matrix, it allows for specific binding and isolation of target enzymes from complex mixtures.

Biochemical Research Tool

As a selective inhibitor, E-64 is utilised in various biochemical and cell biology research applications to study the function and activity of cysteine proteases. It aids in understanding cellular processes regulated by these enzymes.

Enzyme Mechanism Studies

The compound is instrumental in investigating the catalytic mechanisms of cysteine proteases, helping researchers elucidate how these enzymes function at a molecular level.

02 /Properties

Molecular weight	357.41
Empirical formula	C15H27N5O5
Assay	≥99.0% (TLC)
Solubility	H2O: soluble20 mg/mL, clear, colorless
Storage temperature	2-8°C
Application	E-64 is an effective ligand for affinity purification of cysteine proteases. When coupled to a thiolated affinity matrix, binding is no longer irreversible, but specificity is retained.1

03 /Safety & handling

Protective equipment	Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
Water hazard class (WGK, DE)	3

Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.

04 /Identifiers & registry

CAS number

66701-25-5

MDL number

MFCD00080261

Beilstein registry

1405664

05 /Quality & supply

Documentation

Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.

Supply & logistics

Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.

06 /Frequently asked questions

What is E-64 primarily used for?

E-64 is primarily used as an effective ligand for the affinity purification of cysteine proteases and as a tool for studying enzyme mechanisms.

What are the CAS number and molecular formula for E-64?

The CAS number for E-64 is 66701-25-5, and its molecular formula is C15H27N5O5.

What grade and purity does Tech Serve Solutions supply for E-64?

Tech Serve Solutions supplies E-64 with a purity of ≥99.0% (TLC). This product is intended for research purposes and is not represented as USP, BP, or EP grade.

How should E-64 be handled and stored?

E-64 requires careful handling using appropriate personal protective equipment, including eyeshields and gloves. It should be stored at 2-8°C to maintain its stability and efficacy. Refer to the safety data sheet for comprehensive handling guidelines.

Is E-64 a controlled substance?

The provided data does not indicate that E-64 is a controlled substance.

How can I request a quote or sample for E-64?

To request a quote or sample for E-64, please contact our sales team directly through our website or by phone. We are happy to assist with your research needs.

How is E-64 shipped and exported?

Tech Serve Solutions is a specialist global supplier and exporter of fine chemicals. E-64 is carefully packaged and exported in compliance with all relevant regulations to ensure safe and timely delivery worldwide.