E-64d
E-64d (CAS: 88321-09-9), with the molecular formula C17H30N2O5 and a molecular weight of 342.43 g/mol, is a potent inhibitor primarily targeting cysteine proteases, particularly calpains. This compound is widely recognised for its role in biochemical research, specifically in the study of apoptosis and autophagy pathways. E-64d serves as a valuable tool for investigating cellular processes involving protease activity, offering researchers a reliable means to modulate these critical biological functions.
- IUPAC
- (2S,3S)-trans-Epoxysuccinyl-L-leucylamido-3-methylbutane ethyl ester,EST
Cysteine Protease Inhibition
E-64d is a well-established inhibitor of cysteine proteases, with a notable specificity for calpain enzymes. Its application is crucial for dissecting the roles of these proteases in various cellular processes, including cell death and signalling.
Apoptosis Research
The compound is instrumental in studying the mechanisms of apoptosis (programmed cell death). By inhibiting key cysteine proteases involved in apoptotic cascades, researchers can gain insights into the regulation and execution of this vital cellular event.
Autophagy Studies
E-64d is employed to investigate the process of autophagy, a cellular degradation and recycling mechanism. Its use helps researchers understand the interplay between cysteine proteases and autophagy, particularly in conditions of cellular stress.
Cell Signaling Modulator
As a modulator of critical enzyme activity, E-64d is used in research to study cell signalling pathways. It allows for the precise control of protease activity, facilitating a deeper understanding of intracellular communication networks.
| Molecular weight | 342.43 |
|---|---|
| Empirical formula | C17H30N2O5 |
| Solubility | ethanol, DMSO: >10 mg/mL |
| Storage temperature | −20°C |
Hazard statements
- H315Causes skin irritation
- H319Causes serious eye irritation
- H335May cause respiratory irritation
Precautionary statements
- P261Avoid breathing dust, fume, gas or vapours
- P305IF IN EYES
| Protective equipment | dust mask type N95 (US), Eyeshields, Gloves |
|---|---|
| Water hazard class (WGK, DE) | 2 |
| Hazard codes (EU) | Xi |
| Risk statements (R) | 36/37/38 |
| Safety statements (S) | 26-36 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Gene Information | human ... CAPN1(823) |
|---|
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is E-64d used for?
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E-64d is primarily used as a potent cysteine protease inhibitor, particularly for calpains, and is instrumental in research related to apoptosis and autophagy.
What is the CAS number and molecular formula for E-64d?
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The CAS number for E-64d is 88321-09-9, and its molecular formula is C17H30N2O5.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies E-64d as a research-grade chemical. The assay and purity are as specified in the product's Certificate of Analysis; TSS does not represent this product as USP, BP, EP, or pharmacopoeia grade.
How should E-64d be handled and stored?
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E-64d is classified as a warning substance (GHS07) and may cause skin irritation (H315), serious eye irritation (H319), and respiratory irritation (H335). It should be handled with appropriate personal protective equipment, including a dust mask (type N95 US), eyeshields, and gloves. Store in a cool, dry place at -20°C.
Is E-64d a controlled substance?
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The provided data does not indicate that E-64d is a controlled substance.
How can I request a sample or quote for E-64d?
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To request a sample or a quote for E-64d, please contact our sales department through the contact form on our website or call us directly.
How is E-64d typically shipped?
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E-64d is typically shipped according to standard chemical shipping regulations, often with specific temperature controls if required, such as -20°C storage.
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