Cycloheximide
Cycloheximide (CAS: 66-81-9; Formula: C15H23NO4; MW: 281.35) is a potent antibiotic and antifungal agent used in research settings. It functions by inhibiting protein synthesis, making it valuable for selecting resistant strains of yeast and fungi. Cycloheximide also plays a role in controlled studies for protein expression, apoptosis induction, and macrophage clearance in research models. TSS offers this compound for laboratory and research applications.
- IUPAC
- 3-[2-(3,5-Dimethyl-2-oxocyclohexyl)-2-hydroxyethyl]glutarimide,Actidione,Naramycin A
- Synonyms
- 3-[2-(3,5-Dimethyl-2-oxocyclohexyl)-2-hydroxyethyl]glutarimideActidioneNaramycin A
Selection of Resistant Microorganisms
Cycloheximide is employed to selectively isolate strains of yeast and fungi that exhibit resistance to its inhibitory effects. This is crucial in microbiological research for identifying and studying resistant phenotypes.
Protein Synthesis Inhibition
Its mechanism of action involves the controlled inhibition of protein synthesis. This allows researchers to study the turnover of short-lived proteins and facilitate the super-induction of protein expression in experimental systems.
Apoptosis Studies
Cycloheximide is used to induce or facilitate the induction of apoptosis (programmed cell death) in experimental models. It can potentiate apoptosis triggered by death receptors, aiding in cellular research.
Macrophage Research
Studies have shown Cycloheximide's utility in selectively clearing macrophages within atherosclerotic plaques in research contexts. This application aids in understanding macrophage roles in disease progression.
| Molecular weight | 281.35 |
|---|---|
| Empirical formula | C15H23NO4 |
| Concentration | 0.1% |
| Storage temperature | 2-8°C |
| Impurities tested | endotoxin, tested |
| Application | CHX is widely used for selection of CHX-resistant strains of yeast and fungi, controlled inhibition of protein synthesis for detection of short-lived proteins, super-induction of protein expression, and apoptosis induction or facilitation of apoptosis induction by death receptors. It has been shown to selectively clear macrophages in atherosclerotic plaques 1, activate cumulus-free equine oocytes 2, and to have neuroprotective properties 3. |
| Suitability | cell culture tested |
Hazard statements
- H300Fatal if swallowed
- H341Suspected of causing genetic defects
- H360D
- H411Toxic to aquatic life with long-lasting effects
Precautionary statements
- P201Obtain special instructions before use
- P264Wash thoroughly after handling
- P273Avoid release to the environment
- P281Use personal protective equipment as required
- P301IF SWALLOWED
- P308IF exposed or concerned
| Protective equipment | Eyeshields, Faceshields, full-face particle respirator type N100 (US), Gloves, respirator cartridge type N100 (US), type P1 (EN143) respirator filter, type P3 (EN 143) respirator cartridges |
|---|---|
| Transport (UN / ADR) | UN 2811 6.1 / PGI |
| Water hazard class (WGK, DE) | 3 |
| Hazard codes (EU) | T+,N |
| Risk statements (R) | 61-28-51/53-68 |
| Safety statements (S) | 53-45-61 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Gene Information | human ... FKBP1A(2280), PIN1(5300) |
|---|---|
| Shelf Life | (limited shelf life, expiry date on the label) |
| Sterility | 0.2 μm filtered |
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is Cycloheximide used for?
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Cycloheximide is used in research for selecting CHX-resistant yeast and fungi, inhibiting protein synthesis for short-lived protein detection and super-induction of protein expression, and for inducing or facilitating apoptosis. It has also been studied for its effects on macrophages in research models.
What is the CAS number and formula for Cycloheximide?
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The CAS number for Cycloheximide is 66-81-9, and its empirical formula is C15H23NO4.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies Cycloheximide that is cell culture tested and filtered at 0.2 μm, with a concentration of 0.1% and stored at 2-8°C. Purity and grade beyond this are not specified, and TSS does not represent it as USP, BP, or EP grade.
What are the safety and handling requirements for Cycloheximide?
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Cycloheximide is classified as Dangerous with GHS symbols GHS06, GHS08, and GHS09. It carries hazard statements H300 (Fatal if swallowed), H341 (Suspected of causing genetic defects), H360D (May damage the unborn child), and H411 (Toxic to aquatic life with long lasting effects). Appropriate personal protective equipment, including eyeshields, faceshields, gloves, and suitable respiratory protection (type N100 US / P3 EN143), is essential. Handle with extreme caution in a well-ventilated area.
How is Cycloheximide packed and shipped?
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Cycloheximide is shipped as UN 2811, Class 6.1 (Toxic substances), Packing Group I. It requires specific handling and documentation for global export.
How can I request a quote or sample for Cycloheximide?
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To request a quote or a sample of Cycloheximide, please contact our sales team through the website or provide your details via the 'Enquire Now' form.
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