Cholecalciferol
Cholecalciferol (CAS: 67-97-0), with the molecular formula C27H44O and a molecular weight of 384.64 g/mol, is the fat-soluble vitamin D3. It plays a crucial role in calcium and phosphate homeostasis, essential for bone health and immune function. This compound is utilized in various research applications, including cell biology and nutrition research, serving as a vital biochemical standard.
- IUPAC
- (+)-Vitamin D3,7-Dehydrocholesterol activated,Activated 7-dehydrocholesterol,Calciol,Cholecalciferolum
- Synonyms
- Cholecalciferol

Nutrition Research
Cholecalciferol is fundamental in research investigating vitamin D metabolism, calcium absorption, and bone health. It serves as a critical standard for quantifying vitamin D levels in biological samples and food products.
Cell Biology Studies
As a key bioregulator, Cholecalciferol is employed in cell culture experiments to study its effects on cell differentiation, proliferation, and immune responses. Its role in cellular signalling pathways is a significant area of research.
Chemical Synthesis
The compound is used as a starting material or intermediate in the synthesis of various vitamin D analogues and related steroidal compounds. Its stereochemistry makes it valuable in asymmetric synthesis.
Analytical Standard
Cholecalciferol is used as a reference standard in analytical chemistry, particularly in chromatography techniques like HPLC and GC-MS, for accurate identification and quantification.
| Molecular weight | 384.64 |
|---|---|
| Empirical formula | C27H44O |
| Grade | Ph Eur |
| Storage temperature | 2-8°C |
| Melting point | 83-86 °C(lit.) |


Hazard statements
- H301Toxic if swallowed
- H311Toxic in contact with skin
- H330Fatal if inhaled
- H372Causes damage to organs through prolonged exposure
Precautionary statements
- P260Do not breathe dust, fume, gas, mist, vapours or spray
- P280Wear protective gloves, clothing and eye/face protection
- P284Wear respiratory protection
- P301IF SWALLOWED
- P310Immediately call a POISON CENTER or doctor
| Protective equipment | Eyeshields, Faceshields, full-face particle respirator type N100 (US), Gloves, respirator cartridge type N100 (US), type P1 (EN143) respirator filter, type P3 (EN 143) respirator cartridges |
|---|---|
| Transport (UN / ADR) | UN 2811 6.1 / PGII |
| Water hazard class (WGK, DE) | 2 |
| Hazard codes (EU) | T+ |
| Risk statements (R) | 24/25-26-48/25 |
| Safety statements (S) | 28-36/37-45 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Pharmacopeia | testing & handling conforms to Pharmacopeia |
|---|---|
| Feature | standard type fat soluble vitamin |
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is Cholecalciferol used for?
+
Cholecalciferol is primarily used in nutrition research, cell biology studies, chemical synthesis, and as an analytical standard. It is the naturally occurring form of vitamin D3.
What are the CAS number and formula for Cholecalciferol?
+
The CAS number for Cholecalciferol is 67-97-0, and its molecular formula is C27H44O.
What grade and purity does Tech Serve Solutions supply?
+
Tech Serve Solutions supplies Cholecalciferol at the Ph Eur grade, conforming to Pharmacopoeia testing and handling standards. The specific assay is not detailed here but conforms to pharmacopoeial requirements.
How should Cholecalciferol be handled and stored?
+
Cholecalciferol is a toxic substance requiring careful handling. It is labelled with GHS06 and GHS08 symbols and carries a 'Danger' signal word, with hazard statements including H301, H311, H330, and H372. Personal protective equipment such as eyeshields, gloves, and appropriate respirators must be used. Store at 2-8°C.
Is Cholecalciferol a controlled substance?
+
The provided data does not indicate that Cholecalciferol is a controlled substance.
How can I request a sample or quote for Cholecalciferol?
+
To request a sample or a quote for Cholecalciferol, please contact our sales team through the website or by phone. Please specify the quantity required and your delivery location.
How is Cholecalciferol packed and exported?
+
Cholecalciferol is packed and exported in accordance with relevant transport regulations for hazardous materials. It is classified under UN 2811, hazard class 6.1, with a Packing Group of II.
▶ Related products

1-(2-Fluorobenzyl)piperazine
C11H15FN2
Chemical Synthesis
1-(2-Fluorophenyl)biguanide hydrochloride
Chemical Synthesis

1-(2-Fluorophenyl)cyclopropanecarboxylic acid
C10H9FO2
Chemical Synthesis
1-(2-Fluorophenyl)piperazine
C10H13FN2
Chemical Synthesis
1-(2-Fluorophenyl)piperazine monohydrochloride
C10H13FN2 · HCl
Chemical Synthesis
1-(2-Furoyl)-1H-benzotriazole
C11H7N3O2
Chemical Synthesis
1-(2-Furoyl)piperazine
C9H12N2O2
Chemical Synthesis
1-(2-Hydroxy-5-methylphenyl)-3-phenyl-1,3-propanedione
Chemical Synthesis
▶ Explore more

3-Methyl-1,5-di-p-tolyl-1,4-pentazadiene
Chemical Synthesis

3-Methyl-1,5-pentanediol
Chemical Synthesis

3-Methyl-1-(2-methylphenyl)-1H-pyrazol-5-amine
C11H13N3
Chemical Synthesis
3-Methyl-1-(3′-sulfoamidophenyl)-5-pyrazolone
C10H11N3O3S
Chemical Synthesis