Boc-γ-benzyl-Glu-Gly-Arg-7-amido-4-methylcoumarin hydrochloride
Boc-γ-benzyl-Glu-Gly-Arg-7-amido-4-methylcoumarin hydrochloride (CAS: 133448-22-3), with the empirical formula C35H45N7O9 · HCl and a molecular weight of 744.23, is a fluorogenic peptide substrate. It is primarily utilised in biochemical research and enzymatic assays for detecting and quantifying specific protease activities. Its structure allows for sensitive detection of enzyme cleavage, making it a valuable tool in molecular biology and drug discovery.
Enzyme Activity Assays
This compound serves as a sensitive fluorogenic substrate for assays designed to detect and quantify the activity of specific proteases, particularly those that cleave the Glu-Gly-Arg sequence. The release of the 7-amido-4-methylcoumarin fluorophore upon enzymatic cleavage allows for real-time monitoring of enzyme kinetics.
Biochemical Research
Utilised in fundamental biochemical studies to investigate protease function, specificity, and mechanisms of action. Its application aids in understanding complex biological pathways involving proteolytic enzymes.
Drug Discovery and Development
Serves as a tool in screening for novel protease inhibitors or modulators. By measuring enzyme activity changes in the presence of test compounds, researchers can identify potential therapeutic agents.
| Molecular weight | 744.23 |
|---|---|
| Empirical formula | C35H45N7O9 · HCl |
| Assay | ~95% |
| Storage temperature | −20°C |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is Boc-γ-benzyl-Glu-Gly-Arg-7-amido-4-methylcoumarin hydrochloride used for?
+
This compound is a fluorogenic substrate primarily used in biochemical research and enzyme activity assays to detect and quantify the activity of specific proteases.
What are the CAS number and formula for this product?
+
The CAS number is 133448-22-3 and the empirical formula is C35H45N7O9 · HCl.
What grade and purity does Tech Serve Solutions supply?
+
Tech Serve Solutions supplies this product with an assay of approximately 95%. It is suitable for research purposes. We do not represent this product as meeting USP, BP, or EP pharmacopoeial standards.
How should Boc-γ-benzyl-Glu-Gly-Arg-7-amido-4-methylcoumarin hydrochloride be handled?
+
Handle this compound with appropriate personal protective equipment, including eyeshields and gloves. A type N95 (US) or P1 (EN143) respirator filter should be used. Observe standard laboratory safety procedures for handling fine chemicals.
How is this product shipped?
+
This product is typically shipped and exported under conditions appropriate for biochemical reagents, often requiring refrigerated transport. Please contact TSS for specific shipping details and international export compliance.
How can I request a sample or quote?
+
To request a sample or a quotation, please use the 'Request Sample' or 'Request Quote' buttons on the product page, or contact our sales team directly via email or phone.
▶ Related products
![(±)-(E)-4-Ethyl-2-[(E)-hydroxyimino]-5-nitro-3-hexenamide (C8H13N3O4) — chemical structure, CAS 138472-01-2; Biochemicals & Reagents, fine chemical supplied by Tech Serve Solutions](/_next/image?url=https%3A%2F%2Fpub-f4d920c906024441a773a96f7098e551.r2.dev%2Fimages%2Fproducts%2F138472-01-2.png&w=3840&q=75)
(±)-(E)-4-Ethyl-2-[(E)-hydroxyimino]-5-nitro-3-hexenamide
C8H13N3O4
Biochemicals & Reagents![(±)-(E)-4-Ethyl-2-[(Z)-hydroxyimino]-5-nitro-3-hexen-1-yl-nicotinamide (C14H18N4O4) — chemical structure, CAS 162626-99-5; Biochemicals & Reagents, fine chemical supplied by Tech Serve Solutions](/_next/image?url=https%3A%2F%2Fpub-f4d920c906024441a773a96f7098e551.r2.dev%2Fimages%2Fproducts%2F162626-99-5.png&w=3840&q=75)
(±)-(E)-4-Ethyl-2-[(Z)-hydroxyimino]-5-nitro-3-hexen-1-yl-nicotinamide
C14H18N4O4
Biochemicals & Reagents
(±)-2,5-Dimethoxy-4-bromoamphetamine hydrobromide
Biochemicals & Reagents
(±)-2-(p-Methoxyphenoxy)propionic acid
C10H12O4
Biochemicals & Reagents
(±)-2-Amino-6,7-dihydroxy-1,2,3,4-tetrahydronaphthalene hydrobromide
C10H13NO2·HBr
Biochemicals & Reagents
(±)-2-Hydroxydecanoic acid
C10H20O3
Biochemicals & Reagents
(±)-3-Hydroxydecanoic acid
C10H20O3
Biochemicals & Reagents
(±)-3-Hydroxyoctanoic acid
C8H16O3
Biochemicals & Reagents▶ Explore more
3-Methyl-1,5-di-p-tolyl-1,4-pentazadiene
Chemical Synthesis
3-Methyl-1,5-pentanediol
Chemical Synthesis
3-Methyl-1-(2-methylphenyl)-1H-pyrazol-5-amine
C11H13N3
Chemical Synthesis
3-Methyl-1-(3′-sulfoamidophenyl)-5-pyrazolone
C10H11N3O3S
Chemical Synthesis