Acetylthiocholine iodide
Acetylthiocholine iodide (CAS: 1866-15-5) is a bioactive small molecule and a crucial substrate for enzymatic assays. With a molecular weight of 289.18 g/mol and a linear formula of CH3COSCH2CH2N(CH3)3I, it presents as a white powder or crystals. Primarily used for the colorimetric determination of acetylcholinesterase activity, this compound is essential in biochemical research and diagnostics. Tech Serve Solutions supplies Acetylthiocholine iodide with a purity of ≥99.0% (AT).
- IUPAC
- (2-Mercaptoethyl)trimethylammonium iodide acetate

Enzyme Activity Assays
Acetylthiocholine iodide serves as a key substrate for the colorimetric determination of acetylcholinesterase (AChE) activity. Its enzymatic hydrolysis yields a product that can be quantified, making it invaluable for studying enzyme kinetics and inhibitor screening.
Biochemical Research
As a bioactive small molecule, it is utilised in various biochemical and neuroscience research applications. It aids in understanding cholinergic pathways and the function of enzymes involved in neurotransmission.
Diagnostic Reagent
The compound is employed in the development and application of diagnostic kits for detecting acetylcholinesterase levels or activity, particularly relevant in environmental monitoring and toxicology.
| Molecular weight | 289.18 |
|---|---|
| Linear formula | CH3COSCH2CH2N(CH3)3I |
| Assay | ≥99.0% (AT) |
| Form | powder or crystals |
| Colour | white |
| Solubility | H2O: soluble0.1 g/mL, clear, colorless |
| Storage temperature | 2-8°C |
| Melting point | 205-210 °C(lit.)205-210 °C |
| Application | Substrate for the colorimetric determination of acetylcholinesterase activity1. |

Hazard statements
- H301Toxic if swallowed
- H311Toxic in contact with skin
- H315Causes skin irritation
- H319Causes serious eye irritation
- H335May cause respiratory irritation
Precautionary statements
- P261Avoid breathing dust, fume, gas or vapours
- P280Wear protective gloves, clothing and eye/face protection
- P301IF SWALLOWED
- P305IF IN EYES
- P312Call a POISON CENTER if you feel unwell
| Protective equipment | Eyeshields, Faceshields, Gloves, type P2 (EN 143) respirator cartridges |
|---|---|
| Transport (UN / ADR) | UN 2811 6.1 / PGIII |
| Water hazard class (WGK, DE) | 3 |
| Hazard codes (EU) | T |
| Risk statements (R) | 21-25-36/37/38 |
| Safety statements (S) | 26-36 |
| Ignition residue | ≤0.05% |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is Acetylthiocholine iodide primarily used for?
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Acetylthiocholine iodide is primarily used as a substrate for the colorimetric determination of acetylcholinesterase (AChE) activity in biochemical assays and research.
What is the CAS number and formula for Acetylthiocholine iodide?
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The CAS number is 1866-15-5, and its linear formula is CH3COSCH2CH2N(CH3)3I.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies Acetylthiocholine iodide with a minimum purity of ≥99.0% (AT). This is a research-grade chemical and is not supplied as USP, BP, EP, or any other pharmacopoeia grade.
How should Acetylthiocholine iodide be handled and stored?
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Handle Acetylthiocholine iodide with appropriate personal protective equipment, including eyeshields, face shields, gloves, and P2 respirator cartridges, as it is toxic if swallowed or in contact with skin and can cause irritation. Store at 2-8°C to maintain its stability.
How is Acetylthiocholine iodide packed, shipped, and exported?
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We ensure secure and compliant packaging for all our chemicals. Acetylthiocholine iodide is shipped and exported following all relevant international regulations for hazardous materials (UN 2811, 6.1/PGIII).
How can I request a sample or quote for Acetylthiocholine iodide?
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To request a sample or obtain a quote, please contact our sales team through the website or by calling our office, providing the product name and CAS number (1866-15-5).
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