6-(Iodoacetamido)fluorescein
6-(Iodoacetamido)fluorescein (CAS 73264-12-7), with the molecular formula C22H14INO6 and a molecular weight of 515.25, is a fluorescent labelling reagent. This compound is primarily utilised in biological and chemical research for labelling proteins and other biomolecules due to its fluorescent properties. It serves as a valuable tool in cell biology and related research areas for detection and analysis.
- IUPAC
- 6-IAF,N-(3′,6′-Dihydroxy-3-oxospiro[isobenzofuran-1(3H),9′-[9H]xanthen]-6-yl)-2-iodoacetamide
- Synonyms
- N-(3′,6′-Dihydroxy-3-oxospiro[isobenzofuran-1(3H),9′-[9H]xanthen]-6-yl)-2-iodoacetamide, 6-IAF

Fluorescent Labelling
Used for covalent labelling of proteins and other biomolecules containing sulfhydryl groups. The iodoacetamide moiety reacts with thiols, allowing for fluorescent tagging and subsequent detection.
Biochemical Assays
Serves as a reagent in biochemical assays and research, particularly in cell biology and stem cell research, where fluorescent detection is required for tracking or quantifying biological processes.
Molecular Probes
Acts as a fluorescent probe for studying cellular structures, enzyme activity, and biomolecular interactions. Its spectral properties, with absorption at 489 nm and emission around 542 nm, make it suitable for various imaging techniques.
| Molecular weight | 515.25 |
|---|---|
| Empirical formula | C22H14INO6 |
| Assay | ≥95% (HPLC) |
| Storage temperature | 2-8°C |
| Suitability | suitable for fluorescence |
| Absorption | absorption 489 nm in DMSO |
|---|---|
| Fluorescence | λex 488 nm; λem 542 nm in DMF |
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is 6-(Iodoacetamido)fluorescein used for?
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6-(Iodoacetamido)fluorescein is used for fluorescent labelling of biomolecules, particularly proteins containing sulfhydryl groups, and as a molecular probe in biochemical assays and cell biology research.
What are the CAS number and formula for 6-(Iodoacetamido)fluorescein?
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The CAS number for 6-(Iodoacetamido)fluorescein is 73264-12-7, and its empirical formula is C22H14INO6.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies 6-(Iodoacetamido)fluorescein with an assay of ≥95% (HPLC). We do not represent this product as USP, BP, EP, or any other pharmacopoeia grade.
What are the safety and handling considerations for 6-(Iodoacetamido)fluorescein?
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This compound has a WGK Germany rating of 3, indicating it is hazardous to water. It should be handled with appropriate personal protective equipment in a well-ventilated area, and exposure should be avoided. Refer to the Safety Data Sheet for detailed information.
How is 6-(Iodoacetamido)fluorescein shipped and exported by TSS?
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Tech Serve Solutions packs and exports 6-(Iodoacetamido)fluorescein according to international shipping regulations for chemicals, typically requiring storage at 2-8°C.
How can I request a quote or sample for 6-(Iodoacetamido)fluorescein?
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To request a quote or a sample of 6-(Iodoacetamido)fluorescein, please contact our sales team through the TSS website or by phone.
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