6-Carboxyfluorescein N-hydroxysuccinimide ester
6-Carboxyfluorescein N-hydroxysuccinimide ester (CAS: 92557-81-8; Formula: C25H15NO9; MW: 473.39) is a fluorescent labeling reagent. This compound is utilized in biochemical research for the derivatization of primary amines, enabling fluorescent detection and quantification in various biological assays. Its distinct excitation and emission spectra make it valuable for applications requiring sensitive optical detection.
- IUPAC
- 6-Carboxyfluorescein N-succinimidyl ester,6-FAM, SE
Fluorescent Labeling
This succinimidyl ester is employed to covalently attach the fluorescent 6-carboxyfluorescein moiety to primary amine groups found in biomolecules such as proteins and peptides. This facilitates sensitive detection in various biological and analytical techniques.
Biochemical Assays
It serves as a key reagent in developing and performing various biochemical assays where fluorescent detection is crucial. Its specific reactivity allows for targeted labeling in complex biological matrices.
Cell Biology Research
Used in cell biology for labelling cellular components or tracking molecules within cells, leveraging its fluorescent properties for visualisation and analysis.
Diagnostic Reagent Development
The compound's fluorescent characteristics can be exploited in the development of diagnostic kits and reagents that rely on optical detection methods for qualitative or quantitative analysis.
| Molecular weight | 473.39 |
|---|---|
| Empirical formula | C25H15NO9 |
| Storage temperature | −20°C |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Mol Wt | mol wt 473.39 |
|---|---|
| Shipped In | wet ice |
| Fluorescence | λex 492 nm; λem 517 nm (pH 8.0) |
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is 6-Carboxyfluorescein N-hydroxysuccinimide ester used for?
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6-Carboxyfluorescein N-hydroxysuccinimide ester is used for fluorescent labeling of molecules containing primary amine groups, such as proteins and peptides, for detection in biochemical assays and cell biology research.
What are the CAS number and formula for 6-Carboxyfluorescein N-hydroxysuccinimide ester?
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The CAS number is 92557-81-8 and the empirical formula is C25H15NO9.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies this product as a research-grade chemical. The specific purity and assay are detailed in the product's Certificate of Analysis. TSS does not represent this product as USP, BP, or EP grade.
How should 6-Carboxyfluorescein N-hydroxysuccinimide ester be handled and stored?
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Handle with appropriate personal protective equipment, including eyeshields, gloves, and a suitable respirator filter (type N95 or P1). Store at -20°C. It is sensitive to moisture and light.
How is this product shipped?
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6-Carboxyfluorescein N-hydroxysuccinimide ester is shipped with wet ice to maintain its stability during transit.
How can I request a quote or sample?
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To request a quote or sample, please contact our sales team through the TSS website, providing the product name and CAS number (92557-81-8).
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