6-Carboxyfluorescein diacetate
6-Carboxyfluorescein diacetate (CAS 3348-03-6) is a biochemical reagent with the molecular formula C25H16O9. This compound serves as a fluorescent probe, primarily utilized in cell biology to distinguish between viable and apoptotic cells. Its application leverages the enzymatic hydrolysis of the acetate groups within live cells, leading to the accumulation of the fluorescent 6-carboxyfluorescein molecule.
- IUPAC
- 6-CFDA,6-Carboxy-di-O-acetylfluorescein
- Synonyms
- 6-CFDA,6-Carboxy-di-O-acetylfluorescein
Cell Viability Assessment
6-Carboxyfluorescein diacetate is employed to differentiate viable cells from those undergoing apoptosis. Live, non-apoptotic cells accumulate 6-carboxyfluorescein following intracellular esterase activity, allowing for fluorescent detection.
Apoptosis Detection
This reagent aids in the study of apoptosis by enabling the identification of cells that are not yet apoptotic. The presence of intact esterase activity in viable cells distinguishes them from cells in later stages of programmed cell death.
Fluorescent Labeling
As a derivative of fluorescein, it functions as a fluorescent probe. Its emission spectrum is approximately λ<sub>emm</sub> 517 nm with excitation at λ<sub>ex</sub> 492 nm in a 0.1 M Tris buffer at pH 8.0.
| Molecular weight | 460.39 |
|---|---|
| Empirical formula | C25H16O9 |
| Assay | ~95% (HPLC) |
| Storage temperature | −20°C |
| Application | Used to differentiate viable cells from apoptotic cells. Live cells that are not apoptotic accumulate 6-carboxyfluorescein but do not accumulate annexin. Fluorescent wavelengths λex 492 nm; λemm 517 nm in 0.1 M Tris, pH 8.0. |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is 6-Carboxyfluorescein diacetate used for?
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6-Carboxyfluorescein diacetate is used to differentiate viable cells from apoptotic cells. Live cells that are not apoptotic accumulate 6-carboxyfluorescein but do not accumulate annexin. It also serves as a fluorescent probe with excitation at 492 nm and emission at 517 nm.
What is the CAS number and molecular formula for 6-Carboxyfluorescein diacetate?
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The CAS number is 3348-03-6 and the molecular formula is C25H16O9.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies 6-Carboxyfluorescein diacetate with an assay of approximately 95% (HPLC). We do not represent this product as USP, BP, EP or other pharmacopoeia grades.
What are the safety and handling recommendations for 6-Carboxyfluorescein diacetate?
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When handling 6-Carboxyfluorescein diacetate, it is essential to wear eyeshields and gloves. A type N95 (US) or type P1 (EN143) respirator filter should be used if airborne exposure is a risk. The compound has a WGK Germany classification of 3.
How is 6-Carboxyfluorescein diacetate packaged and shipped?
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6-Carboxyfluorescein diacetate is typically stored at −20°C. Tech Serve Solutions is a specialist global supplier and exporter of fine chemicals, pharmaceutical intermediates and laboratory reagents.
How can I request a sample or quote for 6-Carboxyfluorescein diacetate?
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Please contact our sales team to request a sample or a quote for 6-Carboxyfluorescein diacetate. We can assist with export requirements.
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