6-Carboxy-fluorescein diacetate N-succinimidyl ester

CAS 150206-15-8C29H19NO11Biochemicals & Reagents

6-Carboxy-fluorescein diacetate N-succinimidyl ester (CAS: 150206-15-8; Formula: C29H19NO11) is a fluorescent labeling reagent with a molecular weight of 557.46 g/mol. Its succinimidyl ester moiety reacts readily with primary amines, enabling its use in various bioanalytical applications. This compound is valuable for cell tracking, protein modification, and as a fluorescent probe in molecular biology research.

IUPAC: 6-Carboxy-di-O-acetylfluorescein N-succinimidyl ester

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6-Carboxy-fluorescein diacetate N-succinimidyl ester (C29H19NO11) — chemical structure, CAS 150206-15-8; Life Science, fine chemical supplied by Tech Serve Solutions

Biochemicals and ReagentsFluorescein and DerivativesFluorescenceFluorescent LabelsFluorescent ProbesLabelsParticles and StainsMolecular BiologyProtein ModificationProtein Structural Analysis

01 /Applications

Cell Division Tracking

Utilised in flow cytometry for monitoring cell division in both mammalian cells and bacteria. The esterase-cleaved intracellular product is fluorescent, allowing for quantitative tracking of cell proliferation.

Fluorescent Staining of Bacteria

When used with cell permeabilizers, it acts as an effective, non-destructive fluorescent stain for rapid detection of bacterial cells. This method aids in microbiological analysis and diagnostics.

Amine Labeling

The succinimidyl ester group enables spontaneous and irreversible conjugation to free primary amines. This facilitates the fluorescent labeling of proteins and other biomolecules.

02 /Properties

Molecular weight	557.46
Empirical formula	C29H19NO11
Assay	≥90.0% (HPLC)
Grade	for fluorescence
Solubility	DMF: solubleDMSO: soluble
Storage temperature	−20°C
Application	The succinimidyl ester group is capable of spontaneously and irreversibly binding to free amines1. Utilized in flow cytometry experiments for tracking cell division in both mammalian cells and bacteria2,3,4. Used in combination with several cell permeabilizers as an effective, nondestructive fluorescencent stain to quickly detect bacterial cells5.

03 /Safety & handling

Protective equipment	Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
Water hazard class (WGK, DE)	3

Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.

04 /Identifiers & registry

CAS number

150206-15-8

MDL number

MFCD00269772

05 /Additional specifications

Fluorescence	λex 492 nm; λem 517 nm in 0.1 M Tris pH 8.0 (after cleavage by esterase)

06 /Quality & supply

Documentation

Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.

Supply & logistics

Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.

07 /Frequently asked questions

What is 6-Carboxy-fluorescein diacetate N-succinimidyl ester used for?

It is used for fluorescent labeling of amines, cell division tracking in flow cytometry, and as a fluorescent stain for detecting bacterial cells.

What are the CAS number and formula for 6-Carboxy-fluorescein diacetate N-succinimidyl ester?

The CAS number is 150206-15-8 and the empirical formula (Hill notation) is C29H19NO11.

What grade and purity does Tech Serve Solutions supply?

Tech Serve Solutions supplies this product for fluorescence applications with an assay of ≥90.0% (HPLC). We do not represent this product as USP, BP, or EP grade.

How should 6-Carboxy-fluorescein diacetate N-succinimidyl ester be handled and stored?

Handle with appropriate personal protective equipment, including eyeshields, gloves, and a type N95 or P1 respirator filter. Store at -20°C. The product is classified under WGK Germany 3.

Is this product shipped internationally?

Yes, Tech Serve Solutions is a specialist global supplier and exporter of fine chemicals, pharmaceutical intermediates, and laboratory reagents, including this product.