5α-Cholestan-3β-ol
5α-Cholestan-3β-ol (CAS 80-97-7), a sterol lipid with the molecular formula C27H48O, is a derivative of cholesterol. This compound serves as a valuable reagent in biochemical research and laboratory analysis, particularly noted for its application as a standard in High-Performance Liquid Chromatography (HPLC) for lipid profiling. Supplied by Tech Serve Solutions, it is a key component for accurate quantitative and qualitative analysis in various research settings.
- IUPAC
- β-Cholestanol,3β-Hydroxy-5α-cholestane,Dihydrocholesterol

Lipid Analysis Standard
5α-Cholestan-3β-ol is utilised as a reference standard in analytical techniques such as High-Performance Liquid Chromatography (HPLC). This application is crucial for accurate identification and quantification of lipids in complex biological samples.
Biochemical Research Reagent
As a sterol derivative, this compound finds application in various biochemical research studies. It can be used to investigate metabolic pathways, cell membrane structure, and other lipid-related biological processes.
Cell Biology Studies
This sterol is relevant in cell biology research, potentially for studies involving cell membranes, lipid rafts, and cellular signalling where cholesterol derivatives play a role.
| Molecular weight | 388.67 |
|---|---|
| Empirical formula | C27H48O |
| Assay | ≥95% |
| Concentration | 10 mg/mL in chloroform |
| Storage temperature | room temp |
| Application | 5α-Cholestan-3β-ol was used as a standard in lipid analysis using HPLC.1,2 |
| Melting point | 140-142 °C(lit.) |
Precautionary statements
- P261Avoid breathing dust, fume, gas or vapours
- P281Use personal protective equipment as required
- P305IF IN EYES
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Gene Information | human ... POLA1(5422), TOP2A(7153)rat ... Polb(29240) |
|---|
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is 5α-Cholestan-3β-ol primarily used for?
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5α-Cholestan-3β-ol is primarily used as a standard in lipid analysis, particularly in High-Performance Liquid Chromatography (HPLC), and as a reagent in biochemical and cell biology research.
What is the CAS number and molecular formula for 5α-Cholestan-3β-ol?
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The CAS number for 5α-Cholestan-3β-ol is 80-97-7, and its molecular formula is C27H48O.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies 5α-Cholestan-3β-ol with an assay of ≥95%. This product is supplied for research and laboratory use and is not represented as USP, BP, EP, or any other pharmacopoeia grade.
What are the safety precautions when handling 5α-Cholestan-3β-ol?
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When handling 5α-Cholestan-3β-ol, it is essential to wear appropriate personal protective equipment, including eyeshields and gloves. A type N95 (US) or type P1 (EN143) respirator filter should be used if airborne exposure is possible. Handle in accordance with GHS guidelines, with a WGK Germany classification of 3.
How is 5α-Cholestan-3β-ol stored and shipped?
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This compound is typically stored at room temperature. TSS exports globally and can provide specific shipping information upon request. Please consult our shipping policy for international orders.
How can I request a sample or quote for 5α-Cholestan-3β-ol?
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To request a sample or a quote for 5α-Cholestan-3β-ol, please contact our sales team through the website or by phone. Provide the product name and CAS number (80-97-7) for prompt service.
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