5α-Cholestan-3-one
5α-Cholestan-3-one (CAS: 566-88-1; Formula: C27H46O; Molar Mass: 386.65 g/mol) is a sterol derivative characterised by its crystalline form. As a biochemical and lipid compound, it finds application in cell biology and cell signalling research, particularly within studies of cholesterol metabolism and neuroscience. Its specific stereochemistry offers a valuable tool for researchers investigating complex biological pathways.
- IUPAC
- 3-Keto-5α-cholestane
- Synonyms
- 3-Keto-5α-cholestane

Biochemical Research
5α-Cholestan-3-one serves as a valuable biochemical reagent in studies involving lipid metabolism and cellular signalling pathways. Its sterol structure makes it relevant for understanding cholesterol-related biological processes.
Cell Biology Studies
This compound is utilised in cell biology research to investigate the roles of sterols and their derivatives in cellular functions. It can be used to explore how specific lipid structures impact cell behaviour and signalling.
Neuroscience Research
Within neuroscience, 5α-Cholestan-3-one can be employed in studies focusing on lipidomics and their influence on neural function. It is relevant for research into the complex interplay between lipids and the nervous system.
Cholesterol Metabolism Investigations
As a derivative of cholesterol, this compound is a key tool for researchers examining the pathways and regulation of cholesterol metabolism. Its specific structure allows for targeted studies in this area.
| Molecular weight | 386.65 |
|---|---|
| Empirical formula | C27H46O |
| Form | crystalline |
| Melting point | 128-130 °C(lit.) |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is 5α-Cholestan-3-one primarily used for?
+
5α-Cholestan-3-one is primarily used as a biochemical reagent in research settings, particularly for studies in cell biology, cell signalling, neuroscience, and cholesterol metabolism.
What are the CAS number and chemical formula for 5α-Cholestan-3-one?
+
The CAS number for 5α-Cholestan-3-one is 566-88-1, and its chemical formula is C27H46O.
What grade and purity does Tech Serve Solutions supply for 5α-Cholestan-3-one?
+
Tech Serve Solutions supplies 5α-Cholestan-3-one in a technical/research grade. We do not represent this product as meeting USP, BP, or EP pharmacopoeia standards.
What are the key safety considerations when handling 5α-Cholestan-3-one?
+
When handling 5α-Cholestan-3-one, it is essential to use appropriate personal protective equipment, including eyeshields and gloves. A type N95 (US) or type P1 (EN143) respirator filter should be used if airborne exposure is possible. The compound is classified with WGK Germany 3, indicating a high hazard.
How is 5α-Cholestan-3-one typically shipped and exported by TSS?
+
Tech Serve Solutions, as a global supplier and exporter, ships and exports 5α-Cholestan-3-one following all relevant regulations for chemical transport. Packaging is designed to ensure product integrity and safety during transit.
How can I request a sample or a quote for 5α-Cholestan-3-one?
+
To request a sample or a quote for 5α-Cholestan-3-one, please contact our sales department through the website or by phone, providing your specific requirements.
▶ Related products
![(±)-(E)-4-Ethyl-2-[(E)-hydroxyimino]-5-nitro-3-hexenamide (C8H13N3O4) — chemical structure, CAS 138472-01-2; Biochemicals & Reagents, fine chemical supplied by Tech Serve Solutions](/_next/image?url=https%3A%2F%2Fpub-f4d920c906024441a773a96f7098e551.r2.dev%2Fimages%2Fproducts%2F138472-01-2.png&w=3840&q=75)
(±)-(E)-4-Ethyl-2-[(E)-hydroxyimino]-5-nitro-3-hexenamide
C8H13N3O4
Biochemicals & Reagents![(±)-(E)-4-Ethyl-2-[(Z)-hydroxyimino]-5-nitro-3-hexen-1-yl-nicotinamide (C14H18N4O4) — chemical structure, CAS 162626-99-5; Biochemicals & Reagents, fine chemical supplied by Tech Serve Solutions](/_next/image?url=https%3A%2F%2Fpub-f4d920c906024441a773a96f7098e551.r2.dev%2Fimages%2Fproducts%2F162626-99-5.png&w=3840&q=75)
(±)-(E)-4-Ethyl-2-[(Z)-hydroxyimino]-5-nitro-3-hexen-1-yl-nicotinamide
C14H18N4O4
Biochemicals & Reagents
(±)-2,5-Dimethoxy-4-bromoamphetamine hydrobromide
Biochemicals & Reagents

(±)-2-(p-Methoxyphenoxy)propionic acid
C10H12O4
Biochemicals & Reagents
(±)-2-Amino-6,7-dihydroxy-1,2,3,4-tetrahydronaphthalene hydrobromide
C10H13NO2·HBr
Biochemicals & Reagents
(±)-2-Hydroxydecanoic acid
C10H20O3
Biochemicals & Reagents
(±)-3-Hydroxydecanoic acid
C10H20O3
Biochemicals & Reagents
(±)-3-Hydroxyoctanoic acid
C8H16O3
Biochemicals & Reagents▶ Explore more

3-Methyl-1,5-di-p-tolyl-1,4-pentazadiene
Chemical Synthesis

3-Methyl-1,5-pentanediol
Chemical Synthesis

3-Methyl-1-(2-methylphenyl)-1H-pyrazol-5-amine
C11H13N3
Chemical Synthesis
3-Methyl-1-(3′-sulfoamidophenyl)-5-pyrazolone
C10H11N3O3S
Chemical Synthesis