5α-Cholestane
5α-Cholestane (CAS 481-21-0) is a saturated steroid derivative with the molecular formula C27H48 and a molecular weight of 372.67 g/mol. As a cholesterol derivative, it serves as a valuable reference standard and building block in lipid research and steroid chemistry. Its well-defined structure makes it useful in analytical chemistry and biochemical studies, particularly for understanding steroid metabolism and related pathways.

Analytical Reference Standard
5α-Cholestane is utilised as a reference standard in chromatographic techniques, such as Gas Chromatography (GC), for the identification and quantification of sterols and related compounds in complex biological matrices.
Biochemical Research
This saturated sterol is employed in biochemical studies investigating steroid metabolism, enzyme activity, and the structure-function relationships of lipids within cellular systems.
Organic Synthesis Intermediate
As a structurally defined steroid, 5α-Cholestane can function as a starting material or intermediate in the multi-step synthesis of more complex steroid derivatives for pharmaceutical or research applications.
| Molecular weight | 372.67 |
|---|---|
| Empirical formula | C27H48 |
| Assay | ≥97% (GC) |
| Solubility | Do you have solubility information on this product that you would like to share? |
| Application | Do you have application information on this product that you would like to share? |
| Melting point | 80-82 °C(lit.) |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is 5α-Cholestane used for?
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5α-Cholestane is used as an analytical reference standard in chromatography, in biochemical research for studies on steroid metabolism and lipids, and as an intermediate in organic synthesis.
What is the CAS number and molecular formula for 5α-Cholestane?
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The CAS number is 481-21-0 and the molecular formula is C27H48.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies 5α-Cholestane with an assay of ≥97% (GC). We do not represent this product as USP, BP, EP, or any other pharmacopoeia grade.
How should 5α-Cholestane be handled safely?
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When handling 5α-Cholestane, it is important to wear appropriate personal protective equipment, including eyeshields and gloves. A type N95 (US) or type P1 (EN143) respirator filter should be used if necessary. The compound is classified with a WGK Germany of 3, indicating a high hazard to water.
Is 5α-Cholestane a controlled substance?
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The provided data does not indicate that 5α-Cholestane is a controlled substance.
How is 5α-Cholestane packed, shipped, and exported?
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5α-Cholestane is carefully packed according to industry standards to ensure safe transit. We are experienced in global export and can meet diverse shipping requirements.
How can I request a sample or quote for 5α-Cholestane?
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Please contact our sales team via the website or by telephone to request a sample or a quotation for 5α-Cholestane.
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