5α-Cholest-7-en-3β-ol
5α-Cholest-7-en-3β-ol (CAS: 80-99-9) is a sterol with the empirical formula C27H46O and a molecular weight of 386.65 g/mol. This biochemical compound functions as a lipid and a key intermediate in steroid metabolism. It is commonly employed in biochemical research, particularly in enzymatic assays and studies related to cholesterol metabolism and cell biology.
- IUPAC
- 3β-Hydroxy-5α,7-cholestene,Lathosterol
- Synonyms
- Lathosterol3β-Hydroxy-5α,7-cholestene

Enzymatic Assays
5α-Cholest-7-en-3β-ol serves as a substrate in enzymatic assays designed to investigate plasma cholesterol metabolism. Its use allows for precise measurement and study of enzyme activity involved in sterol processing.
Cellular and Metabolic Studies
This compound is utilised in cell biology research to supplement diets in model organisms, such as *Daphnia galeata*. Such applications help elucidate the effects of sterols on life-history traits and metabolic pathways.
Lipid Research
As a sterol lipid, 5α-Cholest-7-en-3β-ol is a valuable tool for researchers studying lipid profiles, membrane composition, and the broader field of lipidomics.
| Molecular weight | 386.65 |
|---|---|
| Empirical formula | C27H46O |
| Storage temperature | −20°C |
| Application | 5α-Cholest-7-en-3β-ol was used as a substrate in enzymatic assay for plasma cholesterol.1 It was used to supplement diet of Daphnia galeata to study the effect on life-history traits.2 |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is 5α-Cholest-7-en-3β-ol used for?
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5α-Cholest-7-en-3β-ol is used as a substrate in enzymatic assays for plasma cholesterol and in cellular studies to investigate the effects of sterols on life-history traits and metabolic pathways. It is also a valuable reagent for lipid and sterol metabolism research.
What are the CAS number and formula for 5α-Cholest-7-en-3β-ol?
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The CAS number for 5α-Cholest-7-en-3β-ol is 80-99-9, and its empirical formula is C27H46O.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies 5α-Cholest-7-en-3β-ol for research purposes. The specific assay and purity are detailed on the product's Certificate of Analysis. TSS does not represent this product as USP, BP, or EP grade.
What are the safety and handling requirements for 5α-Cholest-7-en-3β-ol?
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Handling 5α-Cholest-7-en-3β-ol requires appropriate personal protective equipment, including eyeshields, gloves, and a type N95 (US) or type P1 (EN143) respirator filter. The compound is classified with a WGK Germany rating of 3, indicating a high hazard to water.
How is 5α-Cholest-7-en-3β-ol packaged and shipped?
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Tech Serve Solutions exports fine chemicals globally, adhering to all necessary regulations for packaging and shipping. Specific packaging details will be provided upon order confirmation.
How can I request a quote or sample for 5α-Cholest-7-en-3β-ol?
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To request a quote or sample of 5α-Cholest-7-en-3β-ol, please contact our sales department through the website or by phone, referencing product CAS number 80-99-9.
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