5-Carboxyfluorescein diacetate
5-Carboxyfluorescein diacetate (CAS: 79955-27-4) is a fluorescent dye precursor with the molecular formula C25H16O9 and a molecular weight of 460.39 g/mol. This compound, often employed in biological research, serves as a substrate that can be cleaved by intracellular esterases to release the fluorescent 5-carboxyfluorescein. Its applications are primarily within cell biology and related staining techniques, offering a means to assess cellular esterase activity and viability.
- IUPAC
- 5-CFDA,5-Carboxy-di-O-acetylfluorescein
- Synonyms
- 5-CFDA5-Carboxy-di-O-acetylfluorescein
Cell Viability Assays
5-Carboxyfluorescein diacetate is widely used to assess cell viability and proliferation. Upon entering living cells, intracellular esterases hydrolyze the acetate groups, releasing the highly fluorescent 5-carboxyfluorescein, which can then be quantified.
Esterase Activity Measurement
This compound serves as a sensitive substrate for detecting and quantifying esterase activity within cells and tissues. The rate of fluorescence development is directly proportional to the esterase enzyme activity present.
Histological Staining
In hematology and histology, 5-Carboxyfluorescein diacetate can be employed as a stain precursor. Its conversion to the fluorescent form aids in the visualisation of specific cellular components or processes during microscopic examination.
| Molecular weight | 460.39 |
|---|---|
| Empirical formula | C25H16O9 |
| Assay | ~95% (HPLC) |
| Storage temperature | −20°C |
| Protective equipment | Eyeshields, Faceshields, full-face particle respirator type N100 (US), Gloves, respirator cartridge type N100 (US), type P1 (EN143) respirator filter, type P3 (EN 143) respirator cartridges |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is 5-Carboxyfluorescein diacetate used for?
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5-Carboxyfluorescein diacetate is primarily used in cell biology for assays related to cell viability, proliferation, and the measurement of intracellular esterase activity. It serves as a fluorescent substrate that, upon hydrolysis by esterases, releases the active fluorescent dye 5-carboxyfluorescein.
What are the CAS number and formula for 5-Carboxyfluorescein diacetate?
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The CAS number for 5-Carboxyfluorescein diacetate is 79955-27-4, and its empirical formula is C25H16O9.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies 5-Carboxyfluorescein diacetate with an assay of approximately 95% (HPLC). This product is suitable for research purposes. TSS does not represent this product as USP, BP, EP, or pharmacopoeia grade.
How should 5-Carboxyfluorescein diacetate be handled and stored?
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Due to its potential hazards, appropriate personal protective equipment including eyeshields, faceshields, and gloves should be worn. Respiratory protection is also recommended. Store the compound at "-20°C" to maintain its stability and efficacy.
How is 5-Carboxyfluorescein diacetate shipped?
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Tech Serve Solutions, as a global supplier and exporter of fine chemicals, exports 5-Carboxyfluorescein diacetate adhering to all necessary regulations for chemical shipments. Specific packaging and shipping details are confirmed upon order.
How can I request a quote or sample?
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To request a quote or a sample of 5-Carboxyfluorescein diacetate, please contact our sales department through the website or by calling our customer service line. Our team will be pleased to assist you with your inquiry.
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