4-Hydroxybenzhydrazide
4-Hydroxybenzhydrazide (CAS: 5351-23-5) is a chemical compound with the molecular formula HOC<sub>6</sub>H<sub>4</sub>CONHNH<sub>2</sub> and a molecular weight of 152.15. This compound is suitable for fluorescence applications, exhibiting specific excitation and emission wavelengths when reacted with 5-hydroxymethyl-furaldehyde zinc acetate. It is commonly utilised in biochemical research and as an enzyme inhibitor.
- IUPAC
- 4-Hydroxybenzoylhydrazine,4-Hydroxybenzoic hydrazide,p-Hydroxybenzoic acid hydrazide
- Synonyms
- 4-Hydroxybenzoylhydrazine4-Hydroxybenzoic hydrazidep-Hydroxybenzoic acid hydrazide

Fluorescence Assays
4-Hydroxybenzhydrazide can be used in fluorescence-based detection methods. It exhibits characteristic fluorescence with excitation at 360 nm and emission at 425 nm when complexed with 5-hydroxymethyl-furaldehyde zinc acetate, making it useful for analytical applications.
Enzyme Inhibition Studies
As an enzyme inhibitor, this compound finds application in biochemical research to study enzyme mechanisms and kinetics. Its specific inhibitory properties can help elucidate biological pathways and enzyme functions.
Biochemical Research Reagent
This chemical serves as a valuable reagent in various biochemical research settings. Its properties make it suitable for a range of experimental protocols, particularly those involving enzymatic processes or fluorescent detection.
| Molecular weight | 152.15 |
|---|---|
| Linear formula | HOC6H4CONHNH2 |
| Assay | ≥97% |
| Suitability | suitable for fluorescence |
| Melting point | 264-266 °C (dec.)(lit.) |

Hazard statements
- H315Causes skin irritation
- H319Causes serious eye irritation
- H335May cause respiratory irritation
Precautionary statements
- P261Avoid breathing dust, fume, gas or vapours
- P305IF IN EYES
| Protective equipment | dust mask type N95 (US), Eyeshields, Gloves |
|---|---|
| Water hazard class (WGK, DE) | 3 |
| Hazard codes (EU) | Xi |
| Risk statements (R) | 36/37/38 |
| Safety statements (S) | 26-36 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Fluorescence | λex 360 nm; λem 425 nm (reacted with 5-hydroxymethyl-furaldehydezinc acetate) |
|---|
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is 4-Hydroxybenzhydrazide used for?
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4-Hydroxybenzhydrazide is primarily used in fluorescence assays and as an enzyme inhibitor in biochemical research. It also serves as a general biochemical reagent.
What are the CAS number and formula for 4-Hydroxybenzhydrazide?
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The CAS number for 4-Hydroxybenzhydrazide is 5351-23-5, and its linear formula is HOC<sub>6</sub>H<sub>4</sub>CONHNH<sub>2</sub>.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies 4-Hydroxybenzhydrazide with an assay of ≥97%. We do not represent this product as USP, BP, EP, or other pharmacopoeia grades.
How should 4-Hydroxybenzhydrazide be handled?
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Handle 4-Hydroxybenzhydrazide with care. It is classified as an irritant to skin, eyes, and the respiratory tract. Recommended personal protective equipment includes a dust mask type N95 (US), eyeshields, and gloves. Avoid breathing dust and ensure adequate ventilation.
Is 4-Hydroxybenzhydrazide a controlled substance?
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Based on the provided data, 4-Hydroxybenzhydrazide is not indicated as a controlled substance.
How are Tech Serve Solutions' chemicals packed and shipped?
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Tech Serve Solutions professionally packs and exports chemicals globally, adhering to all relevant transport regulations to ensure safe and secure delivery.
How can I request a sample or quote for 4-Hydroxybenzhydrazide?
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To request a sample or quote for 4-Hydroxybenzhydrazide, please contact our sales team through the website or by phone.
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