4-Hydrazino-7-nitro-benzofurazan hydrazine adduct
4-Hydrazino-7-nitro-benzofurazan hydrazine adduct (CAS: 131467-87-3) is a chemical compound with the formula C6H5N5O3 · NH2NH2 and a molecular weight of 227.18 g/mol. It functions as a fluorescent reagent primarily utilised for protein labeling and as a pre-column derivatisation agent in chromatographic analyses, particularly High-Performance Liquid Chromatography (HPLC).
- IUPAC
- 7-Nitro-4-benzofurazanyl-hydrazine hydrazine adduct,NBD-H

Fluorescent Protein Labeling
This compound serves as a fluorescent reagent for the specific labeling of proteins. Its fluorescent properties allow for sensitive detection and quantification in various biochemical assays.
Chromatographic Derivatisation
It is employed as a pre-column derivatisation agent for chromatographic procedures, notably HPLC. This application enhances the detectability of analytes, particularly carbohydrates, by introducing a fluorescent tag.
Analytical Chemistry Reagent
Utilised in analytical chemistry for its ability to form fluorescent derivatives, aiding in the detection and analysis of specific functional groups in complex samples.
| Molecular weight | 227.18 |
|---|---|
| Empirical formula | C6H5N5O3 · NH2NH2 |
| Storage temperature | 2-8°C |
| Application | 4-Hydrazino-7-nitro-benzofurazan (HBD) is used as a fluorescent reagent for protein labeling and pre-column derivatization for chromatographic procedures such as HPLC. |
| Suitability | suitable for fluorescence |

Hazard statements
- H228Flammable solid
Precautionary statements
- P210Keep away from heat, sparks and open flames. No smoking
| Protective equipment | dust mask type N95 (US), Eyeshields, Gloves |
|---|---|
| Transport (UN / ADR) | UN 1325 4.1 / PGIII |
| Water hazard class (WGK, DE) | 3 |
| Hazard codes (EU) | E |
| Risk statements (R) | 2 |
| Safety statements (S) | 35 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Fluorescence | λex 445 nm; λem 522 nm in chloroform (after derivatization with formaldehyde) |
|---|
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is 4-Hydrazino-7-nitro-benzofurazan hydrazine adduct used for?
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This compound is primarily used as a fluorescent reagent for protein labeling and as a pre-column derivatisation agent in chromatographic procedures like HPLC. It is suitable for fluorescence applications, with excitation at 445 nm and emission at 522 nm in chloroform after derivatisation with formaldehyde.
What are the CAS number and chemical formula for this product?
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The CAS number is 131467-87-3 and the chemical formula is C6H5N5O3 · NH2NH2.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies this chemical as a research-grade reagent. The specific assay and purity are detailed in the product specifications. We do not represent this product as USP, BP, or EP grade.
How should 4-Hydrazino-7-nitro-benzofurazan hydrazine adduct be handled?
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Based on the GHS classification, this compound is classified as flammable (H228) and requires handling with appropriate personal protective equipment, including a dust mask (type N95 US), eyeshields, and gloves. It should be stored at 2-8°C and kept away from ignition sources (P210).
Is this product available for export?
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Yes, Tech Serve Solutions is a specialist global supplier and exporter of fine chemicals, including 4-Hydrazino-7-nitro-benzofurazan hydrazine adduct.
How can I request a quote or sample?
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To request a quote or a sample of 4-Hydrazino-7-nitro-benzofurazan hydrazine adduct, please contact our sales team through the website or via the provided contact details.
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