4-Fluoro-7-nitrobenzofurazan
4-Fluoro-7-nitrobenzofurazan (CAS: 29270-56-2, C6H2FN3O3) is a chemical compound with a molecular weight of 183.10 g/mol. It is particularly useful as a fluorescent label for amino acids and amines in High-Performance Liquid Chromatography (HPLC) applications. Its inherent fluorescence properties make it a valuable reagent in analytical chemistry and biochemical research for detection and quantification purposes.
- IUPAC
- NBD-F

Fluorescent Labeling in HPLC
Utilised for the fluorescent derivatization of amino acids and primary amines. This enhances their detectability in High-Performance Liquid Chromatography, enabling sensitive quantitative analysis.
Biochemical Analysis
Serves as a fluorescent probe for detecting and quantifying amines and amino acids in biological samples. Its specific reactivity allows for targeted labelling in complex matrices.
Fluorescence Detection Reagent
A valuable tool for applications requiring fluorescence detection, offering distinct excitation and emission wavelengths for robust analytical methods.
| Molecular weight | 183.10 |
|---|---|
| Empirical formula | C6H2FN3O3 |
| Assay | ≥98.0% (HPLC) |
| Storage temperature | 2-8°C |
| Application | Useful for fluorescent labeling of amino acids and amines in HPLC |
| Suitability | suitable for fluorescence |
| Melting point | 52-54 °C(lit.) |

Hazard statements
- H315Causes skin irritation
- H319Causes serious eye irritation
Precautionary statements
- P305IF IN EYES
| Protective equipment | dust mask type N95 (US), Eyeshields, Gloves |
|---|---|
| Flash point | 113 °C / 235.4 °F |
| Water hazard class (WGK, DE) | 3 |
| Hazard codes (EU) | Xi |
| Risk statements (R) | 36/38 |
| Safety statements (S) | 26-36 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Fluorescence | λex 470 nm; λem 528 nm in ethanolλex 470 nm; λem 550 nm (Amino acid adducts) |
|---|
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is 4-Fluoro-7-nitrobenzofurazan used for?
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4-Fluoro-7-nitrobenzofurazan is primarily used for the fluorescent labelling of amino acids and amines in HPLC. It acts as a fluorescent probe for sensitive detection and quantification in biochemical and analytical applications.
What is the CAS number and chemical formula for 4-Fluoro-7-nitrobenzofurazan?
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The CAS number is 29270-56-2, and the empirical formula is C6H2FN3O3.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies 4-Fluoro-7-nitrobenzofurazan with an assay of ≥98.0% (HPLC). We do not represent this product as USP, BP, EP, or any other pharmacopoeia grade.
What are the safety considerations for handling 4-Fluoro-7-nitrobenzofurazan?
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This compound is a Warning substance classified with H315 (Causes skin irritation) and H319 (Causes serious eye irritation). Appropriate personal protective equipment, including a dust mask (type N95 US), eyeshields, and gloves, should be used. Refer to the Safety Data Sheet for comprehensive handling information.
How is 4-Fluoro-7-nitrobenzofurazan packaged and shipped?
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Tech Serve Solutions, a global supplier and exporter, ensures appropriate packaging for safe transport and storage. Specific details regarding packaging quantities and shipping options are available upon request.
How can I request a sample or quote for 4-Fluoro-7-nitrobenzofurazan?
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To request a sample or a quote, please contact our sales team through the website or by phone. Our experts will be happy to assist you with your specific requirements.
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