3,3′,5,5′-Tetramethylbenzidine dihydrochloride hydrate
3,3′,5,5′-Tetramethylbenzidine dihydrochloride hydrate (CAS 207738-08-7) is a water-soluble derivative of TMB, identified by the linear formula [C<sub>6</sub>H<sub>2</sub>(CH<sub>3</sub>)<sub>2</sub>-4-NH<sub>2</sub>]<sub>2</sub>·2HCl·xH<sub>2</sub>O and a molecular weight of 313.27 g/mol (anhydrous basis). This compound serves as a convenient chromogenic substrate for the detection of peroxidase activity, particularly in enzyme-linked immunosorbent assays (ELISA). Its solubility enhances ease of use in aqueous biological systems.
- IUPAC
- TMB dihydrochloride
- Synonyms
- 5′-Tetramethylbenzidine dihydrochloride hydrateTMB dihydrochloride hydrate
![3,3′,5,5′-Tetramethylbenzidine dihydrochloride hydrate ([C6H2(CH3)2-4-NH2]2·2HCl·xH2O) — chemical structure, CAS 207738-08-7; Chemistry, fine chemical supplied by Tech Serve Solutions](/_next/image?url=https%3A%2F%2Fpub-f4d920c906024441a773a96f7098e551.r2.dev%2Fimages%2Fproducts%2F207738-08-7.png&w=3840&q=75)
Peroxidase Detection Reagent
Used as a sensitive chromogenic substrate for detecting peroxidase activity in biochemical assays. Its water-soluble nature simplifies preparation and application in ELISA and other enzyme-based detection methods.
Chromogenic Substrate for Immunoassays
A key component in enzyme-linked immunoassays (ELISA) for its ability to produce a coloured product upon enzymatic oxidation by peroxidase. Facilitates the detection and quantification of antigens or antibodies.
Laboratory Reagent
A versatile reagent for general laboratory synthesis and research. Employed in various analytical procedures requiring a water-soluble TMB derivative.
| Molecular weight | 313.27 (anhydrous basis) |
|---|---|
| Linear formula | [-C6H2(CH3)2-4-NH2]2·2HCl·xH2O |
| Assay | 97% |
| Storage temperature | 2-8°C |
| Application | Convenient water-soluble form of TMB for determination of peroxidase. |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is 3,3′,5,5′-Tetramethylbenzidine dihydrochloride hydrate used for?
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3,3′,5,5′-Tetramethylbenzidine dihydrochloride hydrate (CAS 207738-08-7) is primarily used as a water-soluble chromogenic substrate for the detection of peroxidase activity, particularly in enzyme-linked immunosorbent assays (ELISA).
What is the CAS number and formula for this product?
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The CAS number for 3,3′,5,5′-Tetramethylbenzidine dihydrochloride hydrate is 207738-08-7. Its linear formula is [C<sub>6</sub>H<sub>2</sub>(CH<sub>3</sub>)<sub>2</sub>-4-NH<sub>2</sub>]<sub>2</sub>·2HCl·xH<sub>2</sub>O.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies this product with an assay of 97%. This is typically considered a technical or research grade; we do not represent it as USP, BP, EP, or other pharmacopoeia grades.
What are the safety and handling requirements?
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This compound requires the use of Eyeshields and Gloves. A type N95 (US) or type P1 (EN143) respirator filter should be used. The WGK (Germany) is 3, indicating a high hazard to water.
How is this chemical typically packed, shipped, and exported?
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Tech Serve Solutions packs and ships this chemical according to standard chemical handling protocols and international export regulations, ensuring product integrity and compliance. Storage at 2-8°C is recommended.
How can I request a sample or quote?
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To request a sample or obtain a quotation, please contact the Tech Serve Solutions sales team through our website or direct inquiry channels.
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