3,3′,5,5′-Tetramethylbenzidine
3,3′,5,5′-Tetramethylbenzidine (CAS 54827-17-7) is an organic compound with the molecular formula C16H20N2. It serves as a non-carcinogenic alternative to benzidine, commonly employed as a peroxidase substrate in enzyme-linked immunosorbent assays (ELISA). Its ability to produce a soluble coloured product upon enzymatic oxidation makes it valuable in various biochemical detection methods.
- IUPAC
- BM blue,Sure Blue TMB,TMB Blotting Plus,TMB substrate,TMB
- Synonyms
- 5,5′-Tetramethylbenzidine54827-17-7[-C6H2(CH3)2-4-NH2]2
Peroxidase Substrate in ELISA
3,3′,5,5′-Tetramethylbenzidine is widely used as a substrate for horseradish peroxidase in ELISA. It yields a pale blue soluble product upon oxidation, which can be quantified spectrophotometrically.
Blood Detection
This compound is a sensitive and specific reagent for the detection of blood in various forensic and clinical applications.
Assay of Hemoglobin and Peroxidases
It is employed in the quantitative assay of hemoglobin and the detection and quantification of peroxidase activity in biological samples.
| Molecular weight | 240.34 |
|---|---|
| Linear formula | [-C6H2(CH3)2-4-NH2]2 |
| Assay | ≥99% |
| Storage temperature | 2-8°C |
| Application | 3,3′,5,5′-Tetramethylbenzidine (TMB) is a noncarcinogenic substitute (Ames test negative) for benzidine1 suitable as peroxidase substrate for use in ELISA procedures. The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with 2 M H2SO4 (resulting in a yellow color), and read at 450 nm. A sensitive and specific reagent for the detection of blood,2 assay of hemoglobin,3,4,5 assay of peroxidases.6,7,8 |
| Melting point | 168-171 °C(lit.) |
Hazard statements
- H315Causes skin irritation
- H319Causes serious eye irritation
- H335May cause respiratory irritation
Precautionary statements
- P261Avoid breathing dust, fume, gas or vapours
- P305IF IN EYES
| Protective equipment | dust mask type N95 (US), Eyeshields, Faceshields, Gloves |
|---|---|
| Water hazard class (WGK, DE) | 3 |
| Hazard codes (EU) | Xi |
| Risk statements (R) | 36/37/38 |
| Safety statements (S) | 26-36 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is 3,3′,5,5′-Tetramethylbenzidine used for?
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3,3′,5,5′-Tetramethylbenzidine is primarily used as a peroxidase substrate in ELISA, for the detection of blood, and in the assay of hemoglobin and peroxidases.
What are the CAS number and formula for 3,3′,5,5′-Tetramethylbenzidine?
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The CAS number is 54827-17-7 and the molecular formula is C16H20N2.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies 3,3′,5,5′-Tetramethylbenzidine with an assay of ≥99%. We do not represent this product as USP, BP, or EP grade.
How should 3,3′,5,5′-Tetramethylbenzidine be handled?
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Handling requires appropriate personal protective equipment, including a dust mask (N95), eyeshields, face shields, and gloves. Ensure adequate ventilation to avoid dust inhalation, as it can cause skin irritation, eye irritation, and respiratory irritation (H315-H319-H335).
How is 3,3′,5,5′-Tetramethylbenzidine supplied and exported?
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Tech Serve Solutions is a global supplier and exporter of fine chemicals, and 3,3′,5,5′-Tetramethylbenzidine is available for export.
How can I request a quote or sample for 3,3′,5,5′-Tetramethylbenzidine?
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Please contact our sales team to request a quote or a sample of 3,3′,5,5′-Tetramethylbenzidine.
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