3,3′,5,5′-Tetramethylbenzidine
3,3′,5,5′-Tetramethylbenzidine (CAS 54827-17-7), molecular formula 5, is a high-purity chemical supplied by Tech Serve Solutions for chemical synthesis applications, to pharmacopoeia specification (USP, BP, EP). Every batch is tested and ships with a Certificate of Analysis; MSDS and technical data sheets are available on request. Tech Serve Solutions has supplied laboratory reagents, fine chemicals and pharmaceutical intermediates worldwide since 1998.
- IUPAC
- BM blue,Sure Blue TMB,TMB Blotting Plus,TMB substrate,TMB
- Synonyms
- 5′-Tetramethylbenzidine54827-17-7[-C6H2(CH3)2-4-NH2]2
| Molecular weight | 240.34 |
|---|---|
| Linear formula | [-C6H2(CH3)2-4-NH2]2 |
| Assay | ≥99% |
| Storage temperature | 2-8°C |
| Application | 3,3′,5,5′-Tetramethylbenzidine (TMB) is a noncarcinogenic substitute (Ames test negative) for benzidine1 suitable as peroxidase substrate for use in ELISA procedures. The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with 2 M H2SO4 (resulting in a yellow color), and read at 450 nm. A sensitive and specific reagent for the detection of blood,2 assay of hemoglobin,3,4,5 assay of peroxidases.6,7,8 |
| Melting point | 168-171 °C(lit.) |
Hazard statements
- H315Causes skin irritation
- H319Causes serious eye irritation
- H335May cause respiratory irritation
Precautionary statements
- P261Avoid breathing dust, fume, gas or vapours
- P305IF IN EYES
| Protective equipment | dust mask type N95 (US), Eyeshields, Faceshields, Gloves |
|---|---|
| Water hazard class (WGK, DE) | 3 |
| Hazard codes (EU) | Xi |
| Risk statements (R) | 36/37/38 |
| Safety statements (S) | 26-36 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
Documentation
Supplied to USP, BP and EP standards. Every batch ships with a Certificate of Analysis (assay, identity, purity); MSDS and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is 3,3′,5,5′-Tetramethylbenzidine?
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3,3′,5,5′-Tetramethylbenzidine (CAS 54827-17-7), molecular formula 5, is a high-purity chemical supplied by Tech Serve Solutions for chemical synthesis applications, to USP, BP and EP standards.
What grades of 3,3′,5,5′-Tetramethylbenzidine does Tech Serve Solutions supply?
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Tech Serve Solutions supplies 3,3′,5,5′-Tetramethylbenzidine in USP, BP, EP and reagent/AR grades. Custom specifications for nutraceutical or specialty pharmaceutical applications are available — contact our technical sales team.
What is the CAS number and molecular formula of 3,3′,5,5′-Tetramethylbenzidine?
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3,3′,5,5′-Tetramethylbenzidine has CAS number 54827-17-7 and molecular formula 5. Full specifications appear in the table above and in the Certificate of Analysis.
What is the typical minimum order quantity and lead time for 3,3′,5,5′-Tetramethylbenzidine?
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Sample quantities are available for technical evaluation; bulk MOQ depends on grade and packaging. In-stock material typically ships in 7–10 working days; custom-spec orders take 3–4 weeks. Tech Serve Solutions ships worldwide with full export documentation.
Do you provide a Certificate of Analysis (CoA) with each shipment?
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Yes. Every batch of 3,3′,5,5′-Tetramethylbenzidine ships with a Certificate of Analysis covering assay, identity and purity tests, plus pharmacopoeia conformance where applicable. MSDS and TDS documents are available on request before order placement.
How should 3,3′,5,5′-Tetramethylbenzidine be stored?
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Store 3,3′,5,5′-Tetramethylbenzidine in its original sealed container in a cool, dry place away from direct sunlight and incompatible materials, in line with the storage guidance on the Safety Data Sheet. Reseal between dispensings.
What is 3,3′,5,5′-Tetramethylbenzidine used for?
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3,3′,5,5′-Tetramethylbenzidine is used in chemical synthesis applications across pharmaceutical, research and industrial settings. Our technical team can advise on suitability for your specific process.
How do I request a quote for 3,3′,5,5′-Tetramethylbenzidine?
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Request a quote with your required grade, quantity and destination via https://www.techservesolutions.in/contact or email info@techservesolutions.in. We respond with specifications, a Certificate of Analysis and pricing.
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