1,3,5-Triphenyltetrazolium formazan

CAS 531-52-2C19H16N4Biochemicals & Reagents

1,3,5-Triphenyltetrazolium formazan (CAS 531-52-2, C19H16N4) is a tetrazolium salt derivative recognised for its utility as a chromogenic substrate in biochemical assays. This compound is particularly valuable for detecting dehydrogenase activity, commonly employed in microbiology and enzymatic studies. With a molecular weight of 300.36 g/mol, it serves as a reliable indicator of cellular respiration and enzyme function. Tech Serve Solutions supplies this reagent for research and laboratory applications.

IUPAC: TTC formazan
Synonyms: 531-52-2C19H16N4TTC formazan

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1,3,5-Triphenyltetrazolium formazan (C19H16N4) — chemical structure, CAS 531-52-2; Life Science, fine chemical supplied by Tech Serve Solutions

Biochemicals and ReagentsDehydrogenaseEnzyme SubstratesEnzymesInhibitorsand SubstratesSubstrates by Enzyme

01 /Applications

Enzyme Activity Assays

Used as a chromogenic substrate to detect and quantify dehydrogenase activity in various biological samples. The reduction of tetrazolium formazan by enzymes produces a coloured formazan product, enabling spectrophotometric analysis.

Microbiology and Cell Viability

Applicable in microbial studies to assess metabolic activity and cell viability. It helps in differentiating between metabolically active and inactive microorganisms based on enzymatic reduction.

Biochemical Research

Serves as a reagent in research laboratories for investigating enzyme kinetics and pathways involving redox reactions. Its sensitivity allows for the study of enzyme inhibitors and activators.

02 /Properties

Molecular weight	300.36
Empirical formula	C19H16N4
Assay	≥90% (UV)
Solubility	dioxane: soluble50 mg/mL, clear

03 /Safety & handling

GHS hazard pictogram: Harmful / irritant (GHS07) — Harmful / irritant

GHS hazard pictogram: Environmental hazard (GHS09) — Environmental hazard

Warning

Hazard statements

H302Harmful if swallowed
H400Very toxic to aquatic life

Precautionary statements

P273Avoid release to the environment

Protective equipment	dust mask type N95 (US), Eyeshields, Gloves
Transport (UN / ADR)	UN 3077 9 / PGIII
Water hazard class (WGK, DE)	3
Hazard codes (EU)	Xn,N
Risk statements (R)	22-50/53
Safety statements (S)	60-61

Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.

04 /Identifiers & registry

CAS number

531-52-2

MDL number

MFCD00020647

Beilstein registry

753057

05 /Quality & supply

Documentation

Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.

Supply & logistics

Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.

06 /Frequently asked questions

What is 1,3,5-Triphenyltetrazolium formazan used for?

1,3,5-Triphenyltetrazolium formazan is primarily used as a chromogenic substrate to detect dehydrogenase activity in biochemical assays, microbiology, and cell viability studies.

What are the CAS number and formula for 1,3,5-Triphenyltetrazolium formazan?

The CAS number is 531-52-2 and the empirical formula is C19H16N4.

What grade and purity does Tech Serve Solutions supply?

Tech Serve Solutions supplies 1,3,5-Triphenyltetrazolium formazan with an assay of ≥90% (UV). This product is intended for research and laboratory use and is not represented as USP, BP, or EP grade.

How should 1,3,5-Triphenyltetrazolium formazan be handled safely?

Handle with appropriate personal protective equipment, including a dust mask (type N95 US), eyeshields, and gloves. Avoid dust formation and ensure good ventilation. The compound is harmful if swallowed and very toxic to aquatic life with long-lasting effects (H302, H400).